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Titolo:
Early postnatal ethanol intubation blunts GABA(A) receptor up-regulation and modifies 3 alpha-hydroxy-5 alpha-pregnan-20-one sensitivity in rat MS/DBneurons
Autore:
Hsiao, SH; Acevedo, JL; DuBois, DW; Smith, KR; West, JR; Frye, GD;
Indirizzi:
Texas A&M Univ, Hlth Sci Ctr, Coll Med, Dept Med Pharmacol & Toxicol, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 oxicol, College Stn, TX 77843 USA Texas A&M Univ, Hlth Sci Ctr, Dept Human Anat & Med Neurobiol, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 robiol, College Stn, TX 77843 USA
Titolo Testata:
DEVELOPMENTAL BRAIN RESEARCH
fascicolo: 1, volume: 130, anno: 2001,
pagine: 25 - 40
SICI:
0165-3806(20010923)130:1<25:EPEIBG>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC ACID(A); CEREBELLAR GRANULE CELLS; MESSENGER-RNA EXPRESSION; CENTRAL-NERVOUS-SYSTEM; ALCOHOL EXPOSURE; SEPTAL NEURONS; A RECEPTOR; GABA(A)-ACTIVATED CURRENTS; GABA(A)-RECEPTOR SUBTYPES; NEUROSTEROID MODULATION;
Keywords:
basal forebrain; neurosteroid; Zn2+; fetal alcohol syndrome; development; whole-cell patch clamp electrophysiology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
92
Recensione:
Indirizzi per estratti:
Indirizzo: Frye, GD Texas A&M Univ, Hlth Sci Ctr, Coll Med, Dept Med Pharmacol & Toxicol, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 ollege Stn, TX 77843 USA
Citazione:
S.H. Hsiao et al., "Early postnatal ethanol intubation blunts GABA(A) receptor up-regulation and modifies 3 alpha-hydroxy-5 alpha-pregnan-20-one sensitivity in rat MS/DBneurons", DEV BRAIN R, 130(1), 2001, pp. 25-40

Abstract

Previously we found postnatal binge-like ethanol exposure using an artificial-rearing method in the rat delayed developmental up-regulation of GABA(A) receptors (GABA(A)Rs) in both medial septum/diagonal band (MS/DB) and cerebellar Purkinje neurons. In the present study, the impact of ethanol on developing GABA(A)Rs in MS/DB neurons was further tested under conditions notrequiring anesthesia or maternal deprivation. Nursing rat pups received ethanol (4.5-5.25 g/kg/day) on postnatal days (PD) 4-9, which was administrated manually by oral intragastric intubation. This treatment caused dose-dependent blunting of peak GAB(A), receptor whole cell currents in acutely dissociated MS/DB cells on PD 12-15. The threshold with oral intubation was slightly higher than previously observed for artificial-rearing (4.9 vs. 4.5 g/kg/day). The previously observed reduced sensitivity of GAB(A)Rs. to Zn2+-inhibition after ethanol was not found with the intubation model. In studies only carried out using the intubation method, 3 alpha -hydroxy-5 alpha -pregnan-20-one (3 alpha -OH-DHP) caused an allosteric concentration-dependent potentiation of currents activated by non-saturated concentrations of GABA. A bicuculline sensitive direct activation of GABARs also occurred with higher concentrations of 3 alpha -OH-DHP alone. Ethanol intubation up-regulated allosteric neurosteroid potentiation with low concentrations of GABA, but did not change direct agonist actions of 3 alpha -OH-DHR Finally, 3 alpha -OH-DHP did not prime ethanol insensitive GABA(A)Rs to become sensitivity to acute ethanol potentiation. These results indicate ethanol consistently blunts postnatal GABA(A) receptor up-regulation across early postnatal binge-type ethanol exposure models and may increase positive modulation of GABA(A) receptors by endogenous neurosteroids. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:23:00