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Titolo:
Interaction of N1-substituted adenines with 1-methyladenine receptors of starfish oocytes in induction of maturation
Autore:
Mita, M; Yoshikuni, M; Nagahama, Y; Maekawa, M; Saito, M; Saneyoshi, M;
Indirizzi:
Teikyo Jr Coll, Shibuya Ku, Tokyo 1510092, Japan Teikyo Jr Coll Tokyo Japan 1510092 oll, Shibuya Ku, Tokyo 1510092, Japan Natl Inst Basic Biol, Reprod Biol Lab, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 ki, Aichi 4448585, Japan Teikyo Univ Sci & Technol, Dept Biol Sci, Yamanashi 4090193, Japan Teikyo Univ Sci & Technol Yamanashi Japan 4090193 manashi 4090193, Japan
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 427 - 434
SICI:
1096-4959(200110)130:3<427:IONAW1>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEIOTIC MATURATION; PROMOTING FACTOR; SUBSTANCE; PROTEIN; XENOPUS; BINDING;
Keywords:
1-methyladenine; N1-substituted adenine; receptor; binding assay; agonist; antagonist; oocyte maturation; starfish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Mita, M Teikyo Jr Coll, Shibuya Ku, 6-31-1 Honmachi, Tokyo 1510092, Japan Teikyo Jr Coll 6-31-1 Honmachi Tokyo Japan 1510092 1510092, Japan
Citazione:
M. Mita et al., "Interaction of N1-substituted adenines with 1-methyladenine receptors of starfish oocytes in induction of maturation", COMP BIOC B, 130(3), 2001, pp. 427-434

Abstract

Starfish oocytes are arrested naturally in the late G(2) phase of the first meiotic division. In response to the natural maturation-inducing hormone,1-methyladenine (1-MA), oocytes undergo reinitiation of meiosis with germinal vesicle breakdown. We tested 10 newly synthesized NI-substituted adenines that are 1-MA analogues to analyze, the interaction between 1-MA and itsstereo-specific receptors on the oocyte plasma membranes of the starfish Asterina pectinifera. Among these analogues, 1-(beta -naphthylmethyl)adenine, 1-aminoadenine and 1-(p-nitrobenzyl)adenine, played agonistic roles in the induction of oocyte maturation. 1-(o-Nitrobenzyl)adenine, 1-(m-nitrobenzyl)adenine, 1-phenethyladenine and 1-(p-nitrophenethyl)adenine had antagonist effects on 1-MA-induced oocyte maturation. These agonists and antagonistsbehaved competitively in the binding of [H-3]1-MA to receptors in oocyte cortices. In contrast, 1-(alpha -naphthylmethyl)adenine, 1-(2,4-dinitrobenzyl) adenine and 1-(p-methoxybenzyl)adenine had no effects on oocyte maturation. Our results suggest that regional-specific sterical structures at the N1-site of adenine are important in the interaction between 1-MA and its receptors in oocytes. In addition, a negative charge at the N1-site of adenineis required for binding with the receptors. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:57:58