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Titolo:
Control genes in quantitative molecular biological techniques: the variability of invariance
Autore:
Sturzenbaum, SR; Kille, P;
Indirizzi:
Cardiff Univ, Sch Biosci, BIOSI 2, Cardiff, S Glam, Wales Cardiff Univ Cardiff S Glam Wales iosci, BIOSI 2, Cardiff, S Glam, Wales
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 281 - 289
SICI:
1096-4959(200110)130:3<281:CGIQMB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CIS-TRANS-ISOMERASE; MESSENGER-RNA LEVELS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; HOUSEKEEPING GENES; CYCLOSPORINE-A; BETA-ACTIN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; ELONGATION FACTOR-1-ALPHA; SMOOTH-MUSCLE; CYCLOPHILIN-B;
Keywords:
invariant control; housekeeping; GAPDH; cyclophilin; actin; rRNA;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Sturzenbaum, SR Cardiff Univ, Sch Biosci, BIOSI 2, POB 911, Cardiff, S Glam, Wales Cardiff Univ POB 911 Cardiff S Glam Wales , S Glam, Wales
Citazione:
S.R. Sturzenbaum e P. Kille, "Control genes in quantitative molecular biological techniques: the variability of invariance", COMP BIOC B, 130(3), 2001, pp. 281-289

Abstract

The measurement of transcript levels constitutes the foundation of today'smolecular genetics. Independent of the techniques used, quantifications are generally normalised using invariant control genes to account for sample handling, loading and experimental variation. All of the widely used control genes are evaluated, dissecting different methodological approaches and issues regarding the experimental context (e.g. development and tissue type). Furthermore, the major sources of error are highlighted when applying these techniques. Finally, different approaches undertaken to assess the invariance of control genes are critically analysed to generate a procedure thatwill help to discern the best control for novel experiments. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 23:00:42