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Titolo:
Arrangements of macro- and microchromosomes in chicken cells
Autore:
Habermann, FA; Cremer, M; Walter, J; Kreth, G; von Hase, J; Bauer, K; Wienberg, J; Cremer, C; Cremer, T; Solovei, I;
Indirizzi:
Univ Munich, Inst Anthropol & Human Genet, D-8000 Munich, Germany Univ Munich Munich Germany D-8000 & Human Genet, D-8000 Munich, Germany Univ Heidelberg, Kirchhoff Inst Phys, Heidelberg, Germany Univ HeidelbergHeidelberg Germany hhoff Inst Phys, Heidelberg, Germany
Titolo Testata:
CHROMOSOME RESEARCH
fascicolo: 7, volume: 9, anno: 2001,
pagine: 569 - 584
SICI:
0967-3849(200110)9:7<569:AOMAMI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMOSOME TERRITORIES; DNA-REPLICATION; CPG ISLANDS; ORGANIZATION; DYNAMICS; MAMMALS; NUCLEUS; GENOME; ORDER; PROMETAPHASE;
Keywords:
chicken interphase nuclei; chromosome arrangement; 3D M-FISH; nuclear architecture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Solovei, I Univ Munich, Inst Anthropol & Human Genet, D-8000 Munich, Germany Univ Munich Munich Germany D-8000 net, D-8000 Munich, Germany
Citazione:
F.A. Habermann et al., "Arrangements of macro- and microchromosomes in chicken cells", CHROMOS RES, 9(7), 2001, pp. 569-584

Abstract

Arrangements of chromosome territories in nuclei of chicken fibroblasts and neurons were analysed employing multicolour chromosome painting, laser confocal scanning microscopy and three-dimensional (3D) reconstruction. The chicken karyotype consists of 9 pairs of macrochromosomes and 30 pairs of microchromosomes. Although the latter represent only 23% of the chicken genome they contain almost 50% of its genes. We show that territories of microchromosomes in fibroblasts and neurons were clustered within the centre of the nucleus, while territories of the macrochromosomes were preferentially located towards the nuclear periphery. In contrast to these highly consistentradial arrangements, the relative arrangements of macrochromosome territories with respect to each other (side-by-side arrangements) were variable. Astringent radial arrangement of macro- and microchromosomes was found in mitotic cells. Replication labelling studies revealed a pattern of DNA replication similar to mammalian cell nuclei: gene dense, early replicating chromatin mostly represented by microchromosomes, was located within the nuclear interior, surrounded by a rim of late replicating chromatin. These results support the evolutionary conservation of several features of higher-orderchromatin organization between mammals and birds despite the differences in their karyotypes.

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Documento generato il 13/07/20 alle ore 05:06:45