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Titolo:
Intracellular water motion decreases in apoptotic macrophages after caspase activation
Autore:
Hortelano, S; Garcia-Martin, ML; Cerdan, S; Castrillo, A; Alvarez, A; Bosca, L;
Indirizzi:
Univ Complutense, Inst Bioquim, Fac Farm, Ctr Mixto CSIC UCM, Madrid 28040, Spain Univ Complutense Madrid Spain 28040 Mixto CSIC UCM, Madrid 28040, Spain CSIC, Inst Invest Biomed Alberto Sols, Madrid 28029, Spain CSIC Madrid Spain 28029 Invest Biomed Alberto Sols, Madrid 28029, Spain
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 10, volume: 8, anno: 2001,
pagine: 1022 - 1028
SICI:
1350-9047(200110)8:10<1022:IWMDIA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; OXIDE-DEPENDENT APOPTOSIS; PROGRAMMED CELL-DEATH; NITRIC-OXIDE; GENE-THERAPY; IN-VIVO; MECHANISMS; SPECTROSCOPY; DIFFUSION; RELEASE;
Keywords:
H-1 NMR; apoptosis; macrophage; nitric oxide; caspases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Bosca, L Univ Complutense, Inst Bioquim, Fac Farm, Ctr Mixto CSIC UCM, Madrid 28040, Spain Univ Complutense Madrid Spain 28040 IC UCM, Madrid 28040, Spain
Citazione:
S. Hortelano et al., "Intracellular water motion decreases in apoptotic macrophages after caspase activation", CELL DEAT D, 8(10), 2001, pp. 1022-1028

Abstract

Triggering of the macrophage cell line RAW 264.7 with lipopolysaccharide and interferon-gamma promoted apoptosis that was prevented by inhibitors of type 2 nitric oxide synthase or caspase. Using H-1 NMR analysis, we have investigated the changes of the intracellular transverse relaxation time (T-2) and apparent diffusion coefficient (ADC) as parameters reflecting the rotational and translational motions of water in apoptotic macrophages. T-2 values decreased significantly from 287 to 182 ms in cells treated for 18 h with NO-donors. These changes of T-2 were prevented by caspase inhibitors and were not due to mitochondrial depolarization or microtubule depolymerization. The decrease of the intracellular values of T-2 and ADC in apoptotic macrophages was observed after caspase activation, but preceded phosphatidylserine exposure and nucleosomal DNA cleavage. The changes of water motion were accompanied by an enhancement of the hydrophobic properties of the intracellular milieu, as detected by fluorescent probes. These results indicatethe occurrence of an alteration in the physicochemical properties of intracellular water during the course of apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 23:13:08