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Titolo:
Caspase inhibition supports proper gene expression in ex vivo mouse limb cultures
Autore:
De Valck, D; Luyten, FP;
Indirizzi:
Katholieke Univ Leuven, Univ Hosp, Dept Rheumatol, Lab Skeletal Dev & Joint Disorders, Leuven, Belgium Katholieke Univ Leuven Leuven Belgium Joint Disorders, Leuven, Belgium
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 10, volume: 8, anno: 2001,
pagine: 985 - 994
SICI:
1350-9047(200110)8:10<985:CISPGE>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; IN-VITRO; CHONDROCYTE DIFFERENTIATION; SKELETAL DEVELOPMENT; PEPTIDE INHIBITORS; HAMMERTOE MUTANT; COMMON MEDIATOR; JOINT FORMATION; ORGAN-CULTURE; CHICK-EMBRYOS;
Keywords:
mouse limb outgrowth; cartilage maturation; joint formation; interdigital web regression; caspase inhibition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Luyten, FP Katholieke Univ Leuven, Lab Skeletal Dev & Joint Disorders, Campus Gasthuisberg,Bldg O&N,Herestr 49, B-3000 Leuven, Belgium Katholieke Univ Leuven Campus Gasthuisberg,Bldg O&N,Herestr 49 Leuven Belgium B-3000
Citazione:
D. De Valck e F.P. Luyten, "Caspase inhibition supports proper gene expression in ex vivo mouse limb cultures", CELL DEAT D, 8(10), 2001, pp. 985-994

Abstract

We standardized conditions for ex vivo mouse limb culture to study cartilage maturation and joint formation. We compared 12.5 d.p.c. mouse forelimbs that were cultured either mounted or freely rotating for up to 72 h. Limb outgrowth progressed ex vivo at a variable rate as compared to its development in vivo, spanning approximately 48 h. Although cartilage maturation and joint formation developed grossly normal, aberrant expression of skeletal marker genes was seen. Interestingly, no regression of the interdigital webstook place in mounted cultures, in contrast to limited webbing under freely rotating conditions. Caspase inhibition, by addition of zVAD-fmk to the culture medium of freely rotating limbs, supported proper gene expression associated with skeletal development, and prevented interdigital web regression. Taken together, a freely rotating ex vivo culture for mouse limb outgrowth that is combined with caspase inhibition provides a good model to studycartilage maturation and joint formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:47:16