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Titolo:
The role of E-cadherin in the differentiation of gallbladder cancer cells
Autore:
Mukai, S; Miyazaki, K; Yakushiji, H;
Indirizzi:
Saga Med Sch, Dept Surg, Saga 8498501, Japan Saga Med Sch Saga Japan 8498501 Med Sch, Dept Surg, Saga 8498501, Japan
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 306, anno: 2001,
pagine: 117 - 128
SICI:
0302-766X(200110)306:1<117:TROEIT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLAGEN GEL CULTURE; MAMMARY EPITHELIAL-CELLS; ADULT-RAT HEPATOCYTES; ALPHA-CATENIN; ADHESION MOLECULES; BREAST-CANCER; N-CADHERIN; TRANSFORMING GROWTH-FACTOR-BETA-1; MULTICELLULAR SPHEROIDS; JUNCTIONAL COMPLEX;
Keywords:
gallbladder cancer; morphogenesis; E-cadherin; A-catenin; collagen gel culture; human cell lines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Miyazaki, K Saga Med Sch, Dept Surg, 5-1-1 Nabeshima, Saga 8498501, Japan Saga Med Sch 5-1-1 Nabeshima Saga Japan 8498501 498501, Japan
Citazione:
S. Mukai et al., "The role of E-cadherin in the differentiation of gallbladder cancer cells", CELL TIS RE, 306(1), 2001, pp. 117-128

Abstract

Cell adhesion molecules are essential for development and maintenance of epithelial architecture. To clarify the role of these molecules in the morphology of gallbladder cancers, four human gallbladder cancer cell lines (GB-d1, KMG-C, GBK-1, and G-415) were examined in vitro. They showed noticeablydifferent morphologies in our standard gel cultures (SC). GB-d1 and KMG-C formed cystic and spheroid structures, respectively, which seemed to represent well-differentiated and moderately differentiated cancers, respectively. GBK-1 and G-415 showed branching and "pseudoglandular" structures, respectively, both of which seemed to indicate original dedifferentiated cancers. In floating gel culture (FC), only GB-d1 showed a highly increased tendency toward cyst formation. Expression of E-cadherin and alpha -catenin in thegallbladder cancer cell lines was investigated by Western-blotting analysis. Expression was detected in GB-dl and KMG-C, but not in GBK-1 and G-415 cells. Furthermore, E-cadherin expression in GB-d1 was 1.82 times greater inFC than in SC, while E-cadherin expression levels of KMG-C did not change. Neither GB-d1 nor KMG-C showed any difference in alpha -catenin expressionbetween SC and FC. Immunostaining of GB-d1 revealed that these proteins were localized to the cell membrane. In contrast, heterogeneous localization of these proteins was detected in the spheroid structures of KMG-C, in bothSC and FC. Electronmicroscopic examination revealed that reestablishment of the junctional complex occurred only in GB-d1 cells cultured in FC. The formation of cystic structures in GB-d1 was completely inhibited by an antibody against human E-cadherin. Both expression of E-cadherin and its membranous localization are required for well-differentiated-type morphogenesis ingallbladder cancer cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:21:11