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Titolo:
Chondroitin sulfate proteoglycans in the rat thalamus: expression during postnatal development and correlation with calcium-binding proteins in adults
Autore:
Vitellaro-Zuccarello, L; Meroni, A; Amadeo, A; De Biasi, S;
Indirizzi:
Dipartimento Fisiol & Biochim Gen Elettrofisiol, Sez Istol & Anat Umana, I-20133 Milan, Italy Dipartimento Fisiol & Biochim Gen Elettrofisiol MilanItaly I-20133 taly
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 306, anno: 2001,
pagine: 15 - 26
SICI:
0302-766X(200110)306:1<15:CSPITR>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MONOCLONAL-ANTIBODY CAT-301; LATERAL GENICULATE-NUCLEUS; PERINEURONAL NETS; EXTRACELLULAR-MATRIX; IMMUNOCYTOCHEMICAL LOCALIZATION; RETICULAR NUCLEUS; SOMATOSENSORY THALAMUS; CEREBRAL-CORTEX; SURFACE-ANTIGEN;
Keywords:
calretinin; electron microscopy; glycosaminoglycans; parvalbumin; thalamic reticular nucleus; rat (Wistar);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Vitellaro-Zuccarello, L Dipartimento Fisiol & Biochim Gen Elettrofisiol, Sez Istol & Anat Umana, Via Celoria 26, I-20133 Milan, Italy Dipartimento Fisiol & Biochim Gen Elettrofisiol Via Celoria 26 Milan Italy I-20133
Citazione:
L. Vitellaro-Zuccarello et al., "Chondroitin sulfate proteoglycans in the rat thalamus: expression during postnatal development and correlation with calcium-binding proteins in adults", CELL TIS RE, 306(1), 2001, pp. 15-26

Abstract

Postnatal expression of chondroitin sulfate proteoglycans was studied in the rat thalamus by immunocytochemistry and Western immunoblotting techniques with monoclonal antibodies that recognize carbohydrate epitopes (clones CS-56. 1-B-5, 2-B-6). The complex of the results shows that these antibodiesrecognize mostly nonoverlapping molecules whose expression is regulated during postnatal development. Chondroitin sulfate proteoglycans, recognized by antibody CS-56, and hyaluronan, identified by antibody 1-B-5 after hyaluronidase digestion, are abundant in the neuropil of most thalamic nuclei at the perinatal stage and progressively decrease during the second week of life, attaining levels barely detectable by immunocytochemistry at the end ofthe third week. In adult thalamus, chondroitin sulfate proteoglycans of high molecular mass, bearing glycosaminoglycans unsulfated in the linking region, and recognized by antibody 1-B-5 are confined to perineuronal nets around neurons chiefly localized in thalamic reticular nucleus. The immunoreactvity for antibody 2-B-6, specific for chondroitin-4-sulfate, is low at theperinatal stage and is not detectable in adult thalamus. Double-immunolabeling has shown that, along the rostrocaudal extension of reticular nucleus,the most developed perineuronal nets are associated with a subset of neurons expressing calretinin, and not with parvalbumin-positive neurons, which represent the largest neuronal population of the nucleus. The distribution of perineuronal nets supports the presence, in thalamic reticular nucleus, of neuronal subpopulations with different morphological and physiological features.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:49:45