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Titolo:
Crystal structure of a Y-family DNA polymerase in action: A mechanism for error-prone and lesion-bypass replication
Autore:
Ling, H; Boudsocq, F; Woodgate, R; Yang, W;
Indirizzi:
NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MD USA 20892 D, Mol Biol Lab, NIH, Bethesda, MD 20892 USA NICHHD, Sect DNA Replicat Repair & Mutagenesis, NIH, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892 r & Mutagenesis, NIH, Bethesda, MD 20892 USA
Titolo Testata:
CELL
fascicolo: 1, volume: 107, anno: 2001,
pagine: 91 - 102
SICI:
0092-8674(20011005)107:1<91:CSOAYD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPAIR PROTEIN MUTS; ESCHERICHIA-COLI; MISMATCH REPAIR; GENE ENCODES; LARGE FRAGMENT; THYMINE DIMER; FIDELITY; COMPLEX; IOTA; ETA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Yang, W NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA NIDDKD BethesdaMD USA 20892 iol Lab, NIH, Bethesda, MD 20892 USA
Citazione:
H. Ling et al., "Crystal structure of a Y-family DNA polymerase in action: A mechanism for error-prone and lesion-bypass replication", CELL, 107(1), 2001, pp. 91-102

Abstract

Sulfolobus solfataricus P2 DNA polymerase IV [Dpo4) is a DinB homolog thatbelongs to the recently described Y-family of DNA polymerases, which are best characterized by their low-fidelity synthesis on undamaged DNA templates and propensity to traverse normally replication-blocking lesions. Crystalstructures of Dpo4 in ternary complexes with DNA and an incoming nucleotide, either correct or incorrect, have been solved at 1.7 Angstrom and 2.1 Angstrom resolution, respectively. Despite a conserved active site and a hand-like configuration similar to all known polymerases, Dpo4 makes limited and nonspecific contacts with the replicating base pair, thus relaxing base selection. Dpo4 is also captured in the crystal translocating two template bases to the active site at once, suggesting a possible mechanism for bypassing thymine dimers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:01:30