Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Achieving plant disease resistance by antibody expression
Autore:
Fischer, R; Emans, N; Schillberg, S;
Indirizzi:
Fraunhofer Dept Mol Biotechnol, Inst Umweltchem & Okotoxikol, D-57392 Schmallenberg, Germany Fraunhofer Dept Mol Biotechnol Schmallenberg Germany D-57392 rg, Germany
Titolo Testata:
CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE
fascicolo: 3, volume: 23, anno: 2001,
pagine: 236 - 245
SICI:
0706-0661(200109)23:3<236:APDRBA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGEN-DERIVED RESISTANCE; SINGLE-CHAIN ANTIBODY; TRANSGENIC TOBACCO PLANTS; FUNCTIONAL MONOCLONAL-ANTIBODY; HIGH-AFFINITY ANTIBODIES; MOSAIC-VIRUS; NICOTIANA-TABACUM; IN-VITRO; FILAMENTOUS PHAGE; ESCHERICHIA-COLI;
Keywords:
pathogen-resistant crops; genetic engineering; molecular biotechnology; transgenic plants; antibody expression;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
90
Recensione:
Indirizzi per estratti:
Indirizzo: Fischer, R Rhein Westfal TH Aachen, Inst Mol Biotechnol, Worringerweg 1, D-52074 Aachen, Germany Rhein Westfal TH Aachen Worringerweg 1 Aachen Germany D-52074
Citazione:
R. Fischer et al., "Achieving plant disease resistance by antibody expression", CAN J PL P, 23(3), 2001, pp. 236-245

Abstract

Molecular biotechnology has provided powerful: new measures for the control of crop disease. Crops can now be engineered to be resistant through creation of transgenic plants producing recombinant proteins, pathogen-related proteins, or antisense RNAs that block pathogenesis. Antibody engineering is a novel approach to create pathogen-resistant plants, which is based on the expression of recombinant antibodies (rAbs) or rAb fragments that inactivate pathogens and pathogen proteins. The feasibility of this approach has been shown for viral pathogens, where a high degree of resistance has been obtained. The effectiveness of antibody-based resistance is related to the antibody affinity for the target protein, subcellular targeting of the rAb,and the limitations of current rAb expression technology, such as antibodystability in cellular compartments. Antibody-based resistance can create plants that are resistant to pathogens and do not require the undesirable chemical controls currently used in agriculture. Importantly, recombinant-antibody expression can be optimized to overcome any resistance developed by pathogens and 'pyramided' to create resistance to several pathogens. As moresuitable target proteins are identified in plant pathogens, the use of antibody-based resistance and other traits will help to increase crop yields and reduce agrochemical usage. We anticipate that antibody-based resistance will become a major strategy for the molecular breeding of pathogen-resistant crops and pathogen-resistant plant lines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:34:36