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Titolo:
Guanosine 3 ': 5 '-cyclic monophosphate-dependent pathway alterations in ventricular cardiomyocytes of spontaneously hypertensive rats
Autore:
Mazzetti, L; Ruocco, C; Giovannelli, L; Ciuffi, M; Franchi-Micheli, S; Marra, F; Zilletti, L; Failli, P;
Indirizzi:
Univ Florence, Dept Pharmacol, I-50139 Florence, Italy Univ Florence Florence Italy I-50139 Pharmacol, I-50139 Florence, Italy Univ Florence, Dept Internal Med, I-50139 Florence, Italy Univ Florence Florence Italy I-50139 ternal Med, I-50139 Florence, Italy
Titolo Testata:
BRITISH JOURNAL OF PHARMACOLOGY
fascicolo: 3, volume: 134, anno: 2001,
pagine: 596 - 602
SICI:
0007-1188(200110)134:3<596:G3'5'M>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; CARDIAC MYOCYTES; PROTEIN-KINASE; CA2+ CURRENT; CONTRACTILE RESPONSE; GUANYLYL CYCLASE; GENE-EXPRESSION; CALCIUM WAVES; CGMP KINASE; ACTIVATION;
Keywords:
normotensive Wistar Kyoto rats; spontaneously hypertensive rats; cardiomyocytes; guanosine 3 ': 5 '-cyclic monophosphate-dependent protein kinase I; isoprenaline; SNAP; fura-2 fluorescence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Failli, P Univ Florence, Dept Pharmacol, Viale Pieraccini 6, I-50139 Florence, Italy Univ Florence Viale Pieraccini 6 Florence Italy I-50139 , Italy
Citazione:
L. Mazzetti et al., "Guanosine 3 ': 5 '-cyclic monophosphate-dependent pathway alterations in ventricular cardiomyocytes of spontaneously hypertensive rats", BR J PHARM, 134(3), 2001, pp. 596-602

Abstract

1 We investigated the effect of the NO-donor S-nitroso-N-acetyl-DL-penicillamine (SNAP) on cardiomyocytes isolated from control normotensive Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats.2 Ventricular cardiomyocytes were isolated from SHR and WKY hearts and imaging analysis of fura-2-loaded cells was performed in order to evaluate calcium transient in electrical field paced (0.5 Hz) cells.3 In WKY cardiomyocytes, 1-200 mum SNAP dose-dependently increased cyclic GMP content. In basal conditions, cyclic GMP content of SHR cardiomyocytes was significantly higher than in WKY, but SNAP failed to further increase cyclic GMP over the basal level.4 In control conditions, the DeltaF/F and decay time of the calcium transient were similar in both strains. In WKY cardiomyocytes, SNAP (1-100 mum) reduced the decay time. In SHR cardiomyocytes, SNAP was ineffective. Dibutyryl cyclic GMP (10(-6)-10(-8) M), a membrane permeable cyclic GMP analogue, behaved similarly to SNAP.5 In WKY and SHR cardiomyocytes, 10(-8) m isoprenaline similarly increasedDeltaF/F and decreased the decay time. SNAP and dibutyryl cyclic GMP prevented the effect of isoprenaline in WKY, whereas both molecules were ineffective in SHR cardiomyocytes. In WKY, SNAP effects were blocked by pretreating cells with the cGK inhibitor KT-5823.6 Western blotting analysis of cGK type I showed that the enzyme was expressed in WKY isolated cardiomyocytes, but absent in four out of five SHR preparations.7 We concluded that the low expression of cGKI may determine the lack of NO/cyclic GMP-dependent regulation on calcium transient in SHR cardiomyocytes. This alteration may contribute to the development of heart hypertrophy in hypertensive status.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 08:21:21