Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expression of mast cell tryptase by myeloblasts in a group of patients with acute myeloid leukemia
Autore:
Sperr, WR; Jordan, JH; Baghestanian, M; Kiener, HP; Samorapoompichit, P; Semper, H; Hauswirth, A; Schernthaner, GH; Chott, A; Natter, S; Kraft, D; Valenta, R; Schwartz, LB; Geissler, K; Lechner, K; Valent, P;
Indirizzi:
Univ Vienna, Div Hematol & Hemostaseol, Dept Internal Med 1, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Internal Med 1, A-1090 Vienna, Austria Univ Vienna, Dept Internal Med 3, Div Rheumatol, Inst Histol & Embryol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 stol & Embryol, A-1090 Vienna, Austria Univ Vienna, Dept Clin Pathol, A-1090 Vienna, Austria Univ Vienna ViennaAustria A-1090 pt Clin Pathol, A-1090 Vienna, Austria Univ Vienna, Inst Pathophysiol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 t Pathophysiol, A-1090 Vienna, Austria Virginia Commonwealth Univ, Med Coll Virginia, Div Rheumatol Allergy & Immunol, Dept Internal Med, Richmond, VA 23298 USA Virginia Commonwealth UnivRichmond VA USA 23298 , Richmond, VA 23298 USA
Titolo Testata:
BLOOD
fascicolo: 7, volume: 98, anno: 2001,
pagine: 2200 - 2209
SICI:
0006-4971(20011001)98:7<2200:EOMCTB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; BRITISH COOPERATIVE GROUP; MINIMAL RESIDUAL DISEASE; HUMAN PERIPHERAL-BLOOD; MONOCLONAL-ANTIBODIES; BONE-MARROW; PROGNOSTIC-SIGNIFICANCE; INTRACELLULAR ANTIGENS; INCREASED ANGIOGENESIS; SYSTEMIC-ANAPHYLAXIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Sperr, WR Univ Vienna, Div Hematol & Hemostaseol, Dept Internal Med 1, Waehringer Guertel 18-20, A-1090 Vienna, Austria Univ Vienna Waehringer Guertel 18-20 Vienna Austria A-1090 tria
Citazione:
W.R. Sperr et al., "Expression of mast cell tryptase by myeloblasts in a group of patients with acute myeloid leukemia", BLOOD, 98(7), 2001, pp. 2200-2209

Abstract

alpha- and beta -tryptase genes encode serine proteases that are abundantly expressed by mast cells. Under physiologic conditions other myeloid cellsare virtually tryptase negative. However, tryptases are also expressed In several myeloid leukemia cell lines. In this study, serum total tryptase levels were determined In 150 patients with acute leukemias (de novo acute myeloid leukemia [AML], n = 108; secondary AML, n = 25; acute lymphoid leukemia [ALL], n = 17) by fluoroenzyme immunoassay. In healthy subjects (n = 30), tryptase levels ranged between 2.0 and 12.6 ng/mL. Elevated tryptase levels (> 15) were detected In 42 (39%) of 108 patients with de novo AML and In11 (44%) of 25 patients with secondary AML. No elevated tryptase levels were found in patients with ALL. In de novo AML, elevated tryptase levels were frequently detected in patients with French-American-British classification MO (6 of 9), M2 (9 of 14), M3 (4 of 6), and M4eo (7 of 7), and less frequently in M1 (7 of 20), M4 (6 of 26), M5 (2 of 18), M6 (0 of 5), or M7 (1 of 3). The highest tryptase levels were found in M4eo. Immunohistochemical staining of bone marrow sections with anti-tryptase antibody as well as immunoelectron microscopy revealed tryptase expression in the cytoplasm of myeloblasts. As assessed by Northern blotting and reverse transcriptase-polymerase chain reaction, AML cells expressed a-tryptase messenger RNA (mRNA) butlittle or no beta -tryptase mRNA. In AML patients with elevated serum tryptase before chemotherapy, who entered complete remission, tryptase levels returned to normal or near normal values. Blast cell persistence or regrowthwas associated with a persistently elevated level or recurrent Increase oftryptase. Together, tryptase Is expressed In myeloblasts in a group of AMLand may serve as a useful disease-related marker. (C) 2001 by The AmericanSociety of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 06:33:47