Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Modified silver staining of nucleolar organizer regions to improve the accuracy of image analysis
Autore:
Orrea, SC; Tomasi, VH; Schwint, AE; Itoiz, ME;
Indirizzi:
Univ Buenos Aires, Fac Dent, Dept Oral Pathol, RA-1122 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1122 Aires, DF, Argentina Natl Atom Energy Commiss, Dept Radiobiol, Buenos Aires, DF, Argentina NatlAtom Energy Commiss Buenos Aires DF Argentina Aires, DF, Argentina
Titolo Testata:
BIOTECHNIC & HISTOCHEMISTRY
fascicolo: 2, volume: 76, anno: 2001,
pagine: 67 - 73
SICI:
1052-0295(200103)76:2<67:MSSONO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORS; PROTEINS; AGNOR; VISUALIZATION; EPITHELIUM; LESIONS; MARKERS; TISSUES; NEVI;
Keywords:
AgNOR technique; celloidin; image analysis; nitric acid; silver stain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Itoiz, ME Univ Buenos Aires, Fac Dent, Dept Oral Pathol, MT de Alvear 2142, RA-1122 Buenos Aires, DF, Argentina Univ Buenos Aires MT de Alvear 2142 Buenos Aires DF Argentina RA-1122
Citazione:
S.C. Orrea et al., "Modified silver staining of nucleolar organizer regions to improve the accuracy of image analysis", BIOTECH HIS, 76(2), 2001, pp. 67-73

Abstract

Silver staining of nucleolar organizer regions (NORs) and their subsequentquantification by image analysis are used increasingly in human pathological specimens and experimental models. Because certain conditions determinedby the type of tissue and/or its fixation render AgNOR segmentation for image analysis difficult due to insufficient contrast or nonspecific silver precipitation, we propose three improvements to the original technique to overcome these difficulties. Pretreatment with 7% nitric acid produced very distinct dark brown images of AgNORs on a yellow background. The gradient ofbackground colors allowed easy discrimination of nucleolar, nuclear and cytoplasmic structures. Seven morphometric parameters related to number, sizeand shape of AgNORs were evaluated quantitatively by image analysis on sections pretreated with nitric acid and on adjacent sections treated with citrate buffer in a wet autoclave according to the most widely accepted methodfor image analysis of AgNOR. Both methods yielded similar results. A second improvement was achieved by coating the slides with 7% celloidin solutionin ethyl alcohol-ether prior to AgNOR staining and acid pretreatment. Thiscoating prevented nonspecific silver deposition on argyrophilic bacteria and other tissue debris in human vaginal smears that could make visualizing AgNOR sites difficult. Finally, placing sections face down on the staining solution prevents the formation of nonspecific silver precipitates. These procedures can be applied together or separately according to the requirements of the material to be evaluated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:40:22