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Titolo:
Intracellular and extracellular labeling of neurons in fresh slice preparations combined with multiple tract tracing
Autore:
Kuwabara, N; Olson, EK;
Indirizzi:
Univ Louisville, Sch Med, Dept Anat Sci & Neurobiol, Hlth Sci Ctr, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 Sci Ctr, Louisville, KY 40292 USA
Titolo Testata:
BIOTECHNIC & HISTOCHEMISTRY
fascicolo: 4, volume: 76, anno: 2001,
pagine: 173 - 181
SICI:
1052-0295(200107)76:4<173:IAELON>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERIOR PARAOLIVARY NUCLEUS; LUCIFER-YELLOW; TRAPEZOID BODY; COLLATERAL PROJECTIONS; FLUORESCENT TRACERS; INFERIOR COLLICULUS; BRAIN-SLICES; FIXED-SLICE; GUINEA-PIG; RAT;
Keywords:
anterograde labeling; Biocytin; brainstem auditory pathways; fast blue; Fluoro-Gold; gerbil; HRP; Lucifer yellow; retrograde prelabeling; superior olivary complex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Kuwabara, N Univ Louisville, Sch Med, Dept Anat Sci & Neurobiol, Hlth Sci Ctr, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 isville, KY 40292 USA
Citazione:
N. Kuwabara e E.K. Olson, "Intracellular and extracellular labeling of neurons in fresh slice preparations combined with multiple tract tracing", BIOTECH HIS, 76(4), 2001, pp. 173-181

Abstract

We describe methods for studying axo-dendritic projections, one of the forms of neural connection involved in the complex circuits of the central nervous system, including brainstem auditory pathways. This form of neural connection is often difficult to visualize by conventional tract tracing techniques. Retrogradely identified cells were filled intracellularly with a mixture of fluorescent Lucifer yellow and nonfluorescent HRP in live slice preparations to reveal the detailed morphological features of these cells withspecial attention to the distal dendrite that may receive projections fromsuspected or known input axons. Extracellular or intracellular labeling ofcells with axons that project to the distal dendrite of the identified cells was accomplished in the same live slice preparation. Using a live slice rather than a fixed slice allows accurate, visually controlled placement ofanterograde tracer, which requires living axons for transport, into the source of input to the identified cells within the slice. Live slices also permit one to characterize the identified cells electrophysiologically. Intracellular labeling of cells in a potential source of local input to the identified cells also provides conclusive information concerning with connections of the cells involved.

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Documento generato il 19/01/20 alle ore 14:47:54