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Titolo:
Feasibility of fluorescent detection of pathogens on pork carcasses
Autore:
Fravalo, P; Chanet, JP; Mas, M; Huchet, E; Queguiner, S; Salvat, G;
Indirizzi:
French Agcy Food Safety, Hyg & Qual Poultry & Pork Prod Unit, F-22440 Ploufragan, France French Agcy Food Safety Ploufragan France F-22440 440 Ploufragan, France Ctr Agr & Environm Engn Res Sensors & Proc Agr, Food Ind & Environm Res Unit, F-63172 Aubiere, France Ctr Agr & Environm Engn Res Sensors & Proc AgrAubiere France F-63172 ce
Titolo Testata:
BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT
fascicolo: 9-10, volume: 114, anno: 2001,
pagine: 393 - 396
SICI:
0005-9366(200109/10)114:9-10<393:FOFDOP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROPATHOGENIC ESCHERICHIA-COLI; BUNDLE-FORMING PILUS; SALMONELLA-TYPHIMURIUM; LISTERIA-MONOCYTOGENES; CAMPYLOBACTER-JEJUNI; POULTRY SKIN; CELLS; IMMUNOASSAY; ATTACHMENT; GENE;
Keywords:
meat; rapid measure; Salmonella; Listeria monocytogenes; food safety;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Fravalo, P French Agcy Food Safety, Hyg & Qual Poultry & Pork Prod Unit, Zoopole Beaucemaine BP 53, F-22440 Ploufragan, France French Agcy Food Safety Zoopole Beaucemaine BP 53 Ploufragan France F-22440
Citazione:
P. Fravalo et al., "Feasibility of fluorescent detection of pathogens on pork carcasses", BERL MUN TI, 114(9-10), 2001, pp. 393-396

Abstract

The direct immunofluorescent detection of pathogens on pork skin is evaluated. Calibrate contamination of pork skin with Salmonella Typhimurium (ST) and Listeria monocytogenes (Lm) is developed in 2 h at 4 degreesC. Then a specific indirect immunofluorescent staining protocol is optimized in order to obtain specific and intensive signals able to be detected by electronic cameras (deported microscopy). Despite the individual staining of ST and Lmis possible on pork skin and is specific and bright, the deported microscopy failed to detect these particles. After respectively 3 and 6 h, we obtain micro-colonies of ST and Lm. Due to the limited power of the video cameraused, only the microscope permits the detection on the skin. However, our work gives standard conditions to mime the pathogens contamination and staining directly on a biological matrix such as pork skin, This work is a first step in the development of direct and rapid detection of pathogens on biological matrix.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 23:30:14