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Titolo:
Inhibition of sperm motility does not affect live-dead separation of bull sperm by glass beads
Autore:
Foote, RH;
Indirizzi:
Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 niv, Dept Anim Sci, Ithaca, NY 14853 USA
Titolo Testata:
ASIAN JOURNAL OF ANDROLOGY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 193 - 198
SICI:
1008-682X(200109)3:3<193:IOSMDN>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
WOOL COLUMN FILTRATION; PERCOLL GRADIENT; SEMEN SAMPLES; SPERMATOZOA;
Keywords:
sperm motility; glass sphere filters; silicone; fluorides; inhibition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Foote, RH Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 Anim Sci, Ithaca, NY 14853 USA
Citazione:
R.H. Foote, "Inhibition of sperm motility does not affect live-dead separation of bull sperm by glass beads", ASIAN J AND, 3(3), 2001, pp. 193-198

Abstract

Aim This study was designed to explore factors which influence binding of dead versus live sperm to glass filters. Methods: Multiple semen collections from bulls were used to explore selective filtration of bull sperm as influenced by nonlethal inhibition of sperm motility with fluoride, killing ofsperm by quick-freezing, alteration of the glass surface with silicone, and different intervals of sexual rest between semen collections. Results: A comparison of glass spheres 100, 200 and 390 mum in diameter indicated that200 trri spheres were optimal for selective filtration. Quantitative separation of live from dead sperm was demonstrated with a correlation between the percentage of motile sperm and retention of sperm by the filter of r = -0.87 ( P < 0.05). Up to 0.02 mol/L NaFl did not alter the proportion of sperm retained by the filter despite inhibiting sperm motility during filtration, an inhibition which was reversible. Proportions of live-dead sperm, based upon eosin staining, were unaffected by fluoride. Coating the glass spheres with silicone greatly reduced selective filtration. Dead sperm adherence to glass was reduced and resistance to NaFl inhibition was increased by daily ejaculation versus one-week intervals of sexual rest. Conclusion: These studies indicate that the adherence of sperm to glass is primarily due tosome form of physico-chemical change accompanying death of the sperm cell independent of active sperm motility. This attraction between the sperm plasma membrane and glass is modified by the age of the ejaculated sperm. Thisinformation is useful in evaluating different clinical procedures used forsperm separation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:44:32