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Titolo:
A patch-clamp study on human sperm Cl- channel reassembled into giant liposome
Autore:
Bai, JP; Shi, YL;
Indirizzi:
Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Physiol, Key Lab Neurobiol,Shanghai 200031, Peoples R China Chinese Acad Sci Shanghai Peoples R China 200031 200031, Peoples R China
Titolo Testata:
ASIAN JOURNAL OF ANDROLOGY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 185 - 191
SICI:
1008-682X(200109)3:3<185:APSOHS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; CHLORIDE CHANNELS; LIPID BILAYER; ION-CHANNELS; SINGLE; CONDUCTANCE; MEMBRANE; NEURONS; ACID;
Keywords:
Cl- channel; giant liposome; patch clamp; human sperm membrane; ion channel reassembly;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Shi, YL Chinese Acad Sci, Shanghai Inst Physiol, 320 Yue Yang Rd, Shanghai200031,Peoples R China Chinese Acad Sci 320 Yue Yang Rd Shanghai Peoples R China 200031
Citazione:
J.P. Bai e Y.L. Shi, "A patch-clamp study on human sperm Cl- channel reassembled into giant liposome", ASIAN J AND, 3(3), 2001, pp. 185-191

Abstract

Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl- channels in human sperm membrane. Methods:The membrane proteins extracted from the human sperm were reassembled intoliposome bilayer, and the liposomes were fused into giant liposomes with adiameter more than 10 mum by dehydrationrehydration procedure. The giant liposomes were used to study the Cl- channel activities by patch-clamp technique. Results: By patch clamping the giant liposome in an asymmetric NMDG (N-methyl-D-glucamine)-Cl (bath 100// pipette 200 mmol/L) solution system, three kinds of single-channel events with unit conductances of (74.1 +/- 8.3) pS,. (117.0 +/- 5.7) pS and (144.7 +/- 4.5.) pS, respectively, were detected. Their activities were voltage-dependent and all were blocked by SITS (4-acetamido-4'-isothiocyanato-stilbene-2', 2'-disulfonic acid) in a concentration-dependent manner. By constructing the open and close dwell time distribution histograms and then fitting them with exponential function, two time constants were obtained in both the open and the close states. The burstactivity and conductance substate of the channels were observed. Conclusion: There exist three kinds of Cl- channels with different conductance in human sperm membrane at least.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:56:13