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Titolo:
Mitochondrial perturbations and oxidant stress in lymphocytes from patients undergoing surgery and general anesthesia
Autore:
Delogu, G; Moretti, S; Famularo, G; Marcellini, S; Santini, G; Antonucci, A; Marandola, M; Signore, L;
Indirizzi:
Univ Roma La Sapienza, Inst Anesthesia & Intens Care, Policlin Umberto I, I-00161 Rome, Italy Univ Roma La Sapienza Rome Italy I-00161 Umberto I, I-00161 Rome, Italy Univ Roma La Sapienza, Dept Infect Dis, I-00161 Rome, Italy Univ Roma La Sapienza Rome Italy I-00161 Infect Dis, I-00161 Rome, Italy San Camillo Hosp, Dept Med Sci, Rome, Italy San Camillo Hosp Rome ItalySan Camillo Hosp, Dept Med Sci, Rome, Italy
Titolo Testata:
ARCHIVES OF SURGERY
fascicolo: 10, volume: 136, anno: 2001,
pagine: 1190 - 1196
SICI:
0004-0010(200110)136:10<1190:MPAOSI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; SURGICAL STRESS; THYMOCYTE APOPTOSIS; OXIDATIVE STRESS; IN-VIVO; EXPRESSION; NECROSIS; GLUTATHIONE; ACTIVATION; MECHANISMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Delogu, G Univ Roma La Sapienza, Inst Anesthesia & Intens Care, Policlin Umberto I, Piazzale Aldo Moro 5, I-00161 Rome, Italy Univ Roma La Sapienza Piazzale Aldo Moro 5 Rome Italy I-00161 y
Citazione:
G. Delogu et al., "Mitochondrial perturbations and oxidant stress in lymphocytes from patients undergoing surgery and general anesthesia", ARCH SURG, 136(10), 2001, pp. 1190-1196

Abstract

Background: Previous studies have shown that a profound suppression of immune function transiently occurs in patients who undergo surgery under general anesthesia. The decline in the absolute counts of peripheral blood lymphocytes constitutes a major factor accounting for this immune defect, and recent evidence indicates that apoptosis plays a crucial role in determining postsurgical lymphocytopenia. Hypothesis: An altered oxidation-reduction status of mitochondria may contribute through apoptosis to the loss of lymphocytes following surgical trauma and general anesthesia. Design: We studied 16 patients with American Society of Anesthesiologists'physical status I or II who underwent elective surgery under general anesthesia. The data were collected prospectively. Setting: University hospital. Main Outcome Measures: Samples of peripheral blood were drawn on the day before surgery and at 24 and 96 hours after the operation. Following lymphocyte isolation, the mitochondrial transmembrane potential was assessed by flow cytometry using 3,3'-dihexylocarbocyanine iodide, and stains with hydroethidine and 2'7'-dichlorofluorescein diacetate were used to determine the generation of reactive oxygen species. The labeling of lymphocytes with monobromobimane was used to assess the presence of reduced glutathione. Results: At 24 hours after surgery, we detected a significantly elevated frequency of peripheral blood lymphocytes (P=.002), which incorporated low levels of 3,3'-dihexylocarbo-cyanine iodide, compared with the preoperative period. At this same time point, the frequency of lymphocytes with the hydroethidine- and 2'-7'-dichlorofluorescein diacetate-positive phenotype was elevated compared with baseline levels. Conversely, at 24 hours after surgery, the frequency of cells that stained positive for glutathione was strongly decreased compared with preoperative values. Overall measurements returned to the baseline levels at 96 hours after surgery. Conclusion: The strict association we observed between the overproduction of reactive oxygen species and the disruption of the mitochondrial transmembrane potential supports the view that alterations in mitochondrial energy metabolism, paralleled by the presence of a pro-oxidant oxidation-reductionstatus, could be involved in the accelerated apoptotic loss of lymphocytesfollowing surgical trauma and general anesthesia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:38:38