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Titolo:
Evaluation of Tc-99m-MAMA-chrysamine G as an in vivo probe for amyloidosis
Autore:
Dezutter, NA; Landman, WJM; Jager, PL; de Groot, TJ; Dupont, PJ; Tooten, PCJ; Zekarias, B; Gruys, E; Verbruggen, AM;
Indirizzi:
Katholieke Univ Leuven, Fac Pharmaceut Sci, Lab Radiopharmaceut Chem, B-3001 Louvain, Belgium Katholieke Univ Leuven Louvain Belgium B-3001 m, B-3001 Louvain, Belgium Poultry Hlth Ctr, Anim Hlth Serv, Deventer, Netherlands Poultry Hlth Ctr Deventer Netherlands Hlth Serv, Deventer, Netherlands Univ Groningen Hosp, Dept Nucl Med, NL-9713 EZ Groningen, Netherlands UnivGroningen Hosp Groningen Netherlands NL-9713 EZ ningen, Netherlands Univ Hosp Gasthuisberg, Dept Nucl Med, B-3000 Louvain, Belgium Univ Hosp Gasthuisberg Louvain Belgium B-3000 d, B-3000 Louvain, Belgium Univ Utrecht, Fac Vet Med, Dept Pathol, NL-3584 CL Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CL 3584 CL Utrecht, Netherlands
Titolo Testata:
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
fascicolo: 3, volume: 8, anno: 2001,
pagine: 202 - 214
SICI:
1350-6129(200109)8:3<202:EOTGAA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; P-COMPONENT SCINTIGRAPHY; ALZHEIMERS-DISEASE; AA-AMYLOIDOSIS; PRION PROTEIN; CARDIAC AMYLOIDOSIS; MEDULLARY CARCINOMA; FIBRIL PROTEIN; PRE-ALBUMIN; DEPOSITS;
Keywords:
Congo red; chrysamine G; amyloid; SPECT; technetium-99m; I-123-SAP; in vivo probe;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Verbruggen, AM UZ Gasthuisberg, Herestr 49, B-3000 Louvain, Belgium UZ Gasthuisberg Herestr 49 Louvain Belgium B-3000 Belgium
Citazione:
N.A. Dezutter et al., "Evaluation of Tc-99m-MAMA-chrysamine G as an in vivo probe for amyloidosis", AMYLOID, 8(3), 2001, pp. 202-214

Abstract

To date, systemic amyloidosis is diagnosed histologically using Congo red staining or in vivo using iodine-123 labelled serum amyloid P component (I-123-SAP) scintigraphy. We developed Tc-99m-MAMA-CG, a Tc-99m-labelled derivative of the lipophilic Congo red analogue chrysamine G (CG), as a possiblealternative to I-123-SAP. In vivo Tc-99m-MAMA-CG M-CG scintigraphy, performed in chickens with spontaneous joint amyloidosis, resulted as soon.as 10 min after injection in scintigraphic images showing uptake of activity in amyloid-loaded organs (liver, joints). One of these chickens was studied also with I-123-SAP resulting in scintigraphic images revealing I-123-SAP binding to amyloid deposits in the liver. However, up to 11 h after injection no radioactivity was visible in the amyloid positive joints. In vitro autoradiography, performed on sections of chicken joints with Enterococcus faecalis induced amyloid arthropathy (chjAA), demonstrated the failure of Tc-99m-MMA-CG to bind significantly to amyloid deposits in the presence of 10 muM Congo red. The specificity of Tc-99m-MAMA-CG localisation was also established by the absence of Tc-99m-MAMA-CG binding in non-amyloidotic organs in vitro and in vivo, Tc-99m-MAMA-CG did not show any sign of acute toxicity. These findings establish the usefulness of Tc-99m-MAMA-CG as a non-invasive in vivo diagnostic probe in chickens with amyloid arthropathy and suggest that it may also be applicable to human amyloidosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/09/19 alle ore 22:38:03