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Titolo:
Effect of scopoletin on PC3 cell proliferation and apoptosis
Autore:
Liu, XL; Zhang, L; Fu, XL; Chen, K; Qian, BC;
Indirizzi:
Zhejiang Acad Med Sci, Inst Mat Med, Hangzhou 310013, Peoples R China Zhejiang Acad Med Sci Hangzhou Peoples R China 310013 3, Peoples R China
Titolo Testata:
ACTA PHARMACOLOGICA SINICA
fascicolo: 10, volume: 22, anno: 2001,
pagine: 929 - 933
SICI:
0253-9756(200110)22:10<929:EOSOPC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
BENIGN PROSTATIC HYPERPLASIA;
Keywords:
scopoletin; Lycium barbarum; prostate hyperplasia; cultured cells; cell division; apoptosis; flow cytometry; fluorescence microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, XL Zhejiang Acad Med Sci, Inst Mat Med, Hangzhou 310013, Peoples R China Zhejiang Acad Med Sci Hangzhou Peoples R China 310013 es R China
Citazione:
X.L. Liu et al., "Effect of scopoletin on PC3 cell proliferation and apoptosis", ACT PHAR SI, 22(10), 2001, pp. 929-933

Abstract

Aim: To investigate the effect of scopoletin on cell proliferation and apoptosis of PC3 cells. Methods: Cell growth curve, NM assay, and acid phosphatase activity (ACP) were used to determine cell proliferation. Coomassie brilliant blue assay was used to measure the content of protein in cells. Light microscope, transmission electronmicroscope, and fluorescence microscopewere used to observe scopoletin-induced morphological changes. Apoptosis rate and cell cycle distribution were determined by flow cytometry. Results:The IC50 of scopoletin for inhibiting PC3, PAA, and Hela cell proliferation was (157 +/- 25), (154 +/- 51), and (294 +/- 100) mg/L, respectively. Scopoletin induced a marked time- and concentration-dependent inhibition of PC3 cell proliferation. Scopoletin reduced the protein content and decreased the ACP level in PC3 cells in a concentration-dependent manner. Cells treated by scopoletin showed typical morphologic changes of apoptosis by light microscope, fluorescence microscope, and transmission electronmicroscope. Apoptosis rate was 0.3%, 2.1%, 9.3% and 35% for scopoletin 0, 100, 200, and 400 mg/L, respectively, and cells in G(2) phase decreased markedly after being treated with scopoletin. Conclusion: Scopoletin inhibited PC3 proliferation by inducing apoptosis of PC3 cells.

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Documento generato il 04/04/20 alle ore 01:50:46