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Titolo:
Antibody response in salmonids against the 70 kDa serine protease of Aeromonas salmonicida studied by a monoclonal antibody-based ELISA
Autore:
Wagner, U; Hadge, D; Gudmundsdottir, BK; Nold, K; Drossler, K;
Indirizzi:
Univ Leipzig, Inst Zool, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 Inst Zool, D-04103 Leipzig, Germany Univ Iceland, Inst Expt Pathol, IS-112 Reykjavik, Iceland Univ Iceland Reykjavik Iceland IS-112 Pathol, IS-112 Reykjavik, Iceland Fed Inst Hlth Protect Consumers & Vet Med, D-12277 Berlin, Germany Fed Inst Hlth Protect Consumers & Vet Med Berlin Germany D-12277 Germany
Titolo Testata:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
fascicolo: 1-2, volume: 82, anno: 2001,
pagine: 121 - 135
SICI:
0165-2427(20010928)82:1-2<121:ARISAT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMORAL IMMUNE-RESPONSE; ATLANTIC SALMON; SALAR L; EXTRACELLULAR PROTEASE; SSP ACHROMOGENES; RAINBOW-TROUT; FURUNCULOSIS; ANTIGENS; BINDING; CATIONIZATION;
Keywords:
Aeromonas salmonicida; antibody response; serine protease; epitopes; immunogenicity; ELISA; antigen modification; antigen processing; rainbow trout; brown trout; Atlantic salmon;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Wagner, U Attomol GmbH, Mol Diagnost, Schulweg 6, D-03205 Lipten, Germany Attomol GmbH Schulweg 6 Lipten Germany D-03205 Lipten, Germany
Citazione:
U. Wagner et al., "Antibody response in salmonids against the 70 kDa serine protease of Aeromonas salmonicida studied by a monoclonal antibody-based ELISA", VET IMMUNOL, 82(1-2), 2001, pp. 121-135

Abstract

An antigen-capture enzyme-linked immunosorbent assay (cELISA) based on monoclonal antibodies (mAb) was set up and evaluated for selective detection of salmonid antibody responses to the antigen P1, which is a weakly immunogenic exoprotease of typical Aeromonas salmonicida. This new assay permits a specific determination of anti-protease-antibodies, without antigen purification. Serum antibodies induced by the strongly immunogenic lipopolysaccharide could reliably be discriminated from anti-P1-antibodies. Antibody titres of 45 experimental antisera recorded by cELISA were moderately correlatedwith titres determined by routinely used indirect ELISA (iELISA) by detecting partially different antibody populations (r = 0.753). Substitutions of immunoreactants and confirmatory immunoblotting strongly suggest that the mAb-based assay selectively recognises antibodies directed to epitopes of native protease. A conjugate of inhibited protease and cationised bovine serum albumin (cBSA) was found to engender a significant antiprotease-response in three salmonid species (P < 0.05), whereas the unconjugated antigen and Apoject 1-Fural (R) were proved to be ineffective. Recorded specific antibody titres were as high as 1:381,400, indicating a considerable enhanced immunogenicity of cBSA-conjugated P1 and high assay sensitivity. The established cELISA offers a promising approach to further improvement of monitoring fish humoral immune response to surface accessible epitopes of the immunosuppressive exoprotease, P1, and to scrutinise its protective significance. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:11:56