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Titolo:
Adaptor FYB (Fyn-binding protein) regulates integrinmediated adhesion and mediator release: Differential involvement of the FYBSH3 domain
Autore:
Geng, LP; Pfister, S; Kraeft, SK; Rudd, CE;
Indirizzi:
Harvard Univ, Sch Med, Div Tumor Immunol, Dept Canc Immunol & AIDS,Dana Farber Canc Inst, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 a Farber Canc Inst, Boston, MA 02115 USA Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 st, Dept Canc Biol, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA Harvard Univ BostonMA USA 02115 Sch Med, Dept Med, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 h Med, Dept Pathol, Boston, MA 02115 USA Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Haematol, Sch Med, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med London England W12 0NN gland
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 20, volume: 98, anno: 2001,
pagine: 11527 - 11532
SICI:
0027-8424(20010925)98:20<11527:AF(PRI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFFINITY IGE RECEPTOR; FC-EPSILON-RI; BASOPHILIC LEUKEMIA-CELLS; TYROSINE PHOSPHORYLATION; MAST-CELLS; T-CELLS; SIGNAL TRANSDUCTION; MOLECULAR-CLONING; RBL-2H3 CELLS; KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Rudd, CE Harvard Univ, Sch Med, Div Tumor Immunol, Dept Canc Immunol & AIDS,Dana Farber Canc Inst, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 Canc Inst, Boston, MA 02115 USA
Citazione:
L.P. Geng et al., "Adaptor FYB (Fyn-binding protein) regulates integrinmediated adhesion and mediator release: Differential involvement of the FYBSH3 domain", P NAS US, 98(20), 2001, pp. 11527-11532

Abstract

Aggregation of the high-affinity IgE receptor (Fc epsilon RI) on mast cells activates a tyrosine phosphorylation cascade that is required for adhesion and degranulation events leading to the release of histamine and other inflammatory mediators. The full range of intracellular mediators that regulate this process is unknown. Recent studies have identified a group of immune cell-specific adaptor proteins that include linker for activation of T-cell (LAT), SH2-domain-containing leukocyte protein (SLP-76), and Fyn-T-binding protein (FYB)/SLP-76-associated protein (SLAP). In this study, we demonstrate that FYB can up-regulate integrin-mediated adhesion to fibronectin and mediator release in RBL-2H3 mast cells. The regulation of these two events could be distinguished from each other by the requirement of the FYB SH3 domain in beta -hexosaminidase release, but not adhesion, and the up-regulation of mediator release by FYB in nonadherent cells. Fc epsilon RI aggregation increased FYB tyrosine phosphorylation, whereas confocal immunofluorescence microscopy showed that FYB colocalizes with Factin in membrane ruffles and plaques. our findings identify FYB as a regulator of integrin-mediated adhesion and degranulation events, which, in the case of mast cells, has potential applications to inflammatory and allergic responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 00:44:43