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Titolo:
Contrasting modes of evolution acting on the complex N locus for rust resistance in flax
Autore:
Dodds, PN; Lawrence, GJ; Ellis, JG;
Indirizzi:
CSIRO Plant Ind, Canberra, ACT 2601, Australia CSIRO Plant Ind Canberra ACT Australia 2601 Canberra, ACT 2601, Australia
Titolo Testata:
PLANT JOURNAL
fascicolo: 5, volume: 27, anno: 2001,
pagine: 439 - 453
SICI:
0960-7412(200109)27:5<439:CMOEAO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOWNY MILDEW RESISTANCE; LEUCINE-RICH REPEATS; FOR-GENE CONCEPT; DISEASE-RESISTANCE; NUCLEOTIDE-BINDING; DEATH PROCESS; ARABIDOPSIS; FAMILY; SPECIFICITY; CLUSTERS;
Keywords:
disease resistance; TIR-NBS-LRR; rust; sequence exchange; flax; diversifying selection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Ellis, JG CSIRO Plant Ind, Canberra, ACT 2601, Australia CSIRO Plant Ind Canberra ACT Australia 2601 ACT 2601, Australia
Citazione:
P.N. Dodds et al., "Contrasting modes of evolution acting on the complex N locus for rust resistance in flax", PLANT J, 27(5), 2001, pp. 439-453

Abstract

Three rust resistance specificities, N, N1 and N2, map to the complex N locus of flax. We used a degenerate PCR approach, with primers directed to the nucleotide binding site (NIBS) domain characteristic of many plant resistance genes, to isolate resistance gene analogs (RGAs) from flax. One RGA clone detected RFLPs co-segregating with alleles of the N locus. With this probe we isolated four related genes that occur within a 30kbp region and encode proteins with NBS and leucine-rich repeat (LRR) domains and N-terminal Toll/Interleukin-1 Receptor homology (TIR) domains. One of these four geneswas identified as the N resistance gene by sequence analysis of three mutant alleles and by transgenic expression. We isolated homologous genes from two flax lines containing the N1 or N2 specificities and from flax lines carrying no N locus resistance specificities. Analysis of shared polymorphisms among this set of 18 N locus sequences revealed three groups of genes with independent lineages. Sequence exchanges have only occurred between geneswithin each group, but not between groups. Two of the groups contain only one sequence from each haplotype and probably represent orthologous genes. However, the third group contains two genes from each haplotype. We suggestthat the re-assortment of variation by recombination/gene conversion at this locus is limited by the degree of sequence identity between genes.

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Documento generato il 15/08/20 alle ore 19:07:01