Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as akey determinant for proanthocyanidin accumulation in developing seed
Autore:
Nesi, N; Jond, C; Debeaujon, I; Caboche, M; Lepiniec, L;
Indirizzi:
INRA, Ctr Versailles, Lab Biol Semences, Unite Mixte Rech, F-78026 Versailles, France INRA Versailles France F-78026 te Mixte Rech, F-78026 Versailles, France
Titolo Testata:
PLANT CELL
fascicolo: 9, volume: 13, anno: 2001,
pagine: 2099 - 2114
SICI:
1040-4651(200109)13:9<2099:TATGEA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; FLAVONOID BIOSYNTHESIS; TRANSCRIPTIONAL ACTIVATORS; ANTHOCYANIN BIOSYNTHESIS; REGULATORY GENES; DNA-BINDING; MOLECULAR ANALYSIS; PETUNIA ENCODES; REPEAT PROTEIN; MAIZE C1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Lepiniec, L INRA, Ctr Versailles, Lab Biol Semences, Unite Mixte Rech, Route St Cyr, F-78026 Versailles, France INRA Route St Cyr Versailles France F-78026 ersailles, France
Citazione:
N. Nesi et al., "The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as akey determinant for proanthocyanidin accumulation in developing seed", PL CELL, 13(9), 2001, pp. 2099-2114

Abstract

In Arabidopsis, proanthocyanidins specifically accumulate in the endothelium during early seed development. At least three TRANSPARENT TESTA (TT) genes, TT2, TT8, and TTG1, are necessary for the normal expression of several flavonoid structural genes in immature seed, such as DIHYDROFLAVONOL-4-REDUCTASE and BANYULS (BAN). TTS and TTG1 were characterized recently and foundto code for a basic helix-loop-helix domain transcription factor and a WD-repeat-containing protein, respectively. Here the molecular cloning of the TT2 gene was achieved by T-DNA tagging. TT2 encoded an R2R3 MYB domain protein with high similarity to the rice OsMYB3 protein and the maize COLORLESS1 factor. A TT2-green fluorescent protein fusion protein was located mostlyin the nucleus, in agreement with the regulatory function of the native TT2 protein. TT2 expression was restricted to the seed during early embryogenesis, consistent with BAN expression and the proanthocyanidin deposition profile. Finally, in gain-of-function experiments, TT2 was able to induce ectopic expression of BAN in young seedlings and roots in the presence of a functional TT8 protein. Therefore, our results strongly suggest that stringent spatial and temporal BAN expression, and thus proanthocyanidin accumulation, are determined at least partially by TT2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:14:31