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Titolo:
Abnormal DNA-binding of transcription factors in minimal change nephrotic syndrome
Autore:
Cao, CC; Lu, SG; Dong, C; Zhao, RM;
Indirizzi:
Xu Zhou Med Coll, Affiliated Hosp, Dept Pediat, Xuzhou 221002, Jiangsu, Peoples R China Xu Zhou Med Coll Xuzhou Jiangsu Peoples R China 221002 u, Peoples R China
Titolo Testata:
PEDIATRIC NEPHROLOGY
fascicolo: 10, volume: 16, anno: 2001,
pagine: 790 - 795
SICI:
0931-041X(200110)16:10<790:ADOTFI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; BLOOD MONONUCLEAR-CELLS; EXPERIMENTAL GLOMERULONEPHRITIS; MOLECULAR MECHANISMS; ACTIVATION; EXPRESSION; GAMMA; IL-2; RATS;
Keywords:
minimal change nephrotic syndrome glucocorticoid receptor; nuclear factor kappa B; activator protein 1; electrophoretic mobility shift assay; peripheral blood mononuclear cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, SG Xu Zhou Med Coll, Affiliated Hosp, Dept Pediat, Xuzhou 221002, Jiangsu, Peoples R China Xu Zhou Med Coll Xuzhou Jiangsu Peoples R China 221002 les R China
Citazione:
C.C. Cao et al., "Abnormal DNA-binding of transcription factors in minimal change nephrotic syndrome", PED NEPHROL, 16(10), 2001, pp. 790-795

Abstract

The activation of transcription factors such as nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1) plays an important role in regulating the expression of target genes, including those for cytokines involved inpathogenesis of minimal change nephrotic syndrome (MCNS). The therapeutic effects of glucocorticoids depend on the glucocorticoid receptor (GR) acting on gene transcription and interacting with certain transcription factors. To explore the role of transcription factors in the pathogenesis of MCNS and the therapeutic effects of glucocorticoids, we examined the DNA-binding abilities of NF-kappaB, AP-1, and GR in peripheral blood mononuclear cells (PBMC) from 6 children with MCNS and 6 healthy controls by electrophoretic mobility shift assay (EMSA). NF-kappaB and AP-1 DNA-binding abilities were significantly increased both at baseline and after stimulation by phorbol 12-myristate 13-acetate (TPA) in PBMC from MCNS patients compared with controls, but declined to normal levels after treatment with dexamethasone (DEX). GR DNA-binding abilities were significantly reduced at baseline and aftertreatment with TPA, but were enhanced markedly by DEX. There were strong correlations between urinary protein and the baseline DNA binding ability ofNF-kappaB or AP-1, or GR. These results suggested that the abnormal activation of NF-kappaB and AP-1 and the reduction of GR DNA-binding abilities may be involved in the pathogenesis of MCNS. Inhibition of NF-kappaB and AP-1and enhancement of GR DNA-binding abilities by DEX may form the molecular basis of the effects of glucocorticoids in MCNS.

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Documento generato il 18/01/20 alle ore 21:40:59