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Titolo:
Nociceptin-induced internalization of the ORLI receptor in human neuroblastoma cells
Autore:
Spampinato, S; Di Toro, R; Qasem, AR;
Indirizzi:
Univ Bologna, Inst Pharmacol, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 nst Pharmacol, I-40126 Bologna, Italy
Titolo Testata:
NEUROREPORT
fascicolo: 14, volume: 12, anno: 2001,
pagine: 3159 - 3163
SICI:
0959-4965(20011008)12:14<3159:NIOTOR>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-COUPLED RECEPTORS; MU-OPIOID RECEPTOR; BETA-ARRESTIN; ORPHANIN FQ/NOCICEPTIN; DESENSITIZATION; AGONIST; MEMBRANES; BINDING; LIGAND;
Keywords:
internalization; neuroblastoma; nociceptin; opioid receptor-like I receptor; recycling; SK-N-BE cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Spampinato, S Univ Bologna, Inst Pharmacol, Irnerio 48, I-40126 Bologna, Italy Univ Bologna Irnerio 48 Bologna Italy I-40126 ologna, Italy
Citazione:
S. Spampinato et al., "Nociceptin-induced internalization of the ORLI receptor in human neuroblastoma cells", NEUROREPORT, 12(14), 2001, pp. 3159-3163

Abstract

Nociceptin/orphanin FQ (INC) has been proposed as endogenous ligand of theopioid receptor-like I (ORLI) receptor. We investigated NC-induced internalization and recycling of the ORLI receptor in SK-N-BE human neuroblastoma cells. Internalization was proven by receptor binding assay on viable cells. NC promotes a time- and concentration-dependent internalization of the ORLI receptor (57% of cell surface receptors are lost after 30 min exposure to 1 muM NC) in a clathrin- and ATP-dependent manner. After 30 min exposure to NC, ORLI receptor internalization is partially reversible and recycling is dependent on acid phosphatases. Over-expression of beta -arrestin 2 increases NC-promoted internalization of the ORLI receptor. These events contribute to NC signaling in neuronal cells through sequestration and recycling of the ORLI receptor. NeuroReport 12:3159-3163 (C) 2001 Lippincott Williams& Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:51:38