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Titolo:
Cell-to-cell movement of potato virus X: The role of p12 and p8 encoded bythe second and third open reading frames of the triple gene block
Autore:
Tamai, A; Meshi, T;
Indirizzi:
Kyoto Univ, Dept Bot, Grad Sch Sci, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 d Sch Sci, Sakyo Ku, Kyoto 6068502, Japan
Titolo Testata:
MOLECULAR PLANT-MICROBE INTERACTIONS
fascicolo: 10, volume: 14, anno: 2001,
pagine: 1158 - 1167
SICI:
0894-0282(200110)14:10<1158:CMOPVX>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRIPE MOSAIC-VIRUS; GREEN FLUORESCENT PROTEIN; SIZE-EXCLUSION LIMIT; COAT PROTEIN; SUBCELLULAR-LOCALIZATION; MUTATIONAL ANALYSIS; TOBACCO PLANTS; VIRAL-PROTEINS; 3A PROTEIN; RNA-BETA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Tamai, A Kyoto Univ, Dept Bot, Grad Sch Sci, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 , Sakyo Ku, Kyoto 6068502, Japan
Citazione:
A. Tamai e T. Meshi, "Cell-to-cell movement of potato virus X: The role of p12 and p8 encoded bythe second and third open reading frames of the triple gene block", MOL PL MICR, 14(10), 2001, pp. 1158-1167

Abstract

Potato virus X (PVX) requires three proteins, p25, p12, and p8, encoded bythe triple gene block plus the coat protein (CP) for cell-to-cell movement. When each of these proteins was co-expressed with a cytosolic green fluorescent protein (GFP) in the epidermal cells of Nicotiana benthamiana by themicroprojectile bombardment-mediated gene delivery method, only p12 enhanced diffusion of coexpressed GFP, indicating an ability to alter plasmodesmal permeability. p25, p12, and CP, expressed transiently in the initially infected cells, transcomplemented the corresponding movement-defective mutants to spread through two or more cell boundaries. Thus, these proteins probably move from cell to cell with the genomic RNA. In contrast, p8 only functioned intracellularly and was not absolutely required for cell-to-cell movement. Since overexpression of p12 overcame the p8 deficiency, p8 appears tofacilitate the functioning of p12, presumably by mediating its intracellular trafficking. Considering the likelihood that p12 and p8 are membrane proteins, it is suggested that intercellular as well as intracellular movementof PVX involves a membrane-mediated process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:29:14