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Titolo:
The cyclin-dependent kinase inhibitor roscovitine inhibits RNA synthesis and triggers nuclear accumulation of p53 that is unmodified at Ser15 and Lys382
Autore:
Ljungman, M; Paulsen, MT;
Indirizzi:
Univ Michigan, Dept Radiat Oncol, Div Canc Biol, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 rehens Canc, Ann Arbor, MI 48109 USA Univ Michigan, Sch Med, Mol & Cellular Biol Program, Ann Arbor, MI USA Univ Michigan Ann Arbor MI USA Cellular Biol Program, Ann Arbor, MI USA
Titolo Testata:
MOLECULAR PHARMACOLOGY
fascicolo: 4, volume: 60, anno: 2001,
pagine: 785 - 789
SICI:
0026-895X(200110)60:4<785:TCKIRI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CDK-ACTIVATING KINASE; TRANSCRIPTION FACTOR TFIIH; CARBOXYL-TERMINAL DOMAIN; SUPPRESSOR PROTEIN P53; POLYMERASE-II; DNA-DAMAGE; CELL-CYCLE; INDUCED APOPTOSIS; PHOSPHORYLATION; ELONGATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Ljungman, M Univ Michigan, Dept Radiat Oncol, Div Canc Biol, Ctr Comprehens Canc, 4306CCGC,1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA Univ Michigan 4306 CCGC,1500 E Med Ctr Dr Ann Arbor MI USA 48109
Citazione:
M. Ljungman e M.T. Paulsen, "The cyclin-dependent kinase inhibitor roscovitine inhibits RNA synthesis and triggers nuclear accumulation of p53 that is unmodified at Ser15 and Lys382", MOLEC PHARM, 60(4), 2001, pp. 785-789

Abstract

Roscovitine has been shown to induce the accumulation of the tumor suppressor p53, to arrest cells in the G(1) and G(2)/M phases of the cell cycle, and to induce apoptosis in human cells. Although these cellular effects of roscovitine are thought to be caused directly by its specific inhibition of cyclin-dependent kinases, other mechanisms may contribute as well. In this study, we investigated whether roscovitine interferes with transcription inhuman cells. We have previously shown that blockage of transcription is a trigger for the induction of p53 and apoptosis in human fibroblasts. Here we show that mRNA synthesis is suppressed significantly by roscovitine in human cells. Furthermore, our results suggest that the mechanism by which roscovitine inhibits RNA synthesis involves the inhibition of the phosphorylation of the carboxyl-terminal domain of RNA polymerase II. Cells treated with roscovitine at doses that affected transcription were found to accumulatep53 in the nucleus; curiously, however, the nuclear accumulation of p53 was not accompanied by modifications at either the Ser15 or Lys382 sites of p53. We conclude that roscovitine is a potent inhibitor of RNA synthesis andthat this inhibition may be responsible for the accumulation of nuclear p53.

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Documento generato il 30/11/20 alle ore 07:20:45