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Titolo:
Molecular genetic analysis of the candidate gene for MOD, a locus requiredfor self-incompatibility in Brassica rapa
Autore:
Fukai, E; Nishio, T; Nasrallah, ME;
Indirizzi:
Tohoku Univ, Grad Sch Agr Sci, Lab Plant Breeding & Genet, Aoba Ku, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 Ku, Sendai, Miyagi 9818555, Japan Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 v, Dept Plant Biol, Ithaca, NY 14853 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 3, volume: 265, anno: 2001,
pagine: 519 - 525
SICI:
1617-4615(200105)265:3<519:MGAOTC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
S-LOCUS; ARABIDOPSIS-THALIANA; RECEPTOR KINASE; PROTEIN; STIGMA; PLASMALEMMA; DETERMINANT; AQUAPORINS; CAMPESTRIS; WATER;
Keywords:
Brassica rapa var. Yellow Sarson (YS); self-incompatibility; self-compatibility; MOD; MIP-MOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Nishio, T Tohoku Univ, Grad Sch Agr Sci, Lab Plant Breeding & Genet, Aoba Ku, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 , Miyagi 9818555, Japan
Citazione:
E. Fukai et al., "Molecular genetic analysis of the candidate gene for MOD, a locus requiredfor self-incompatibility in Brassica rapa", MOL GENET G, 265(3), 2001, pp. 519-525

Abstract

7The MIP-MOD (for MOD-locus associated Major Intrinsic Protein) gene encodes an aquaporin-like product, and has been reported to be a candidate for the MOD gene which is required for the self-incompatibility response in Brassica raga. In an antisense suppression experiment designed to investigate the role of MIP-MOD, we found that levels of MIP-MOD mRNA in the stigmas of fourteen antisense transgenics, as well as in the self incompatible cultivar Osome (Osm), were much lower than in the stigmas of the self-incompatibleS-8 homozygous (S-8) strain. Therefore, we analyzed the molecular structure of the MIP-MOD gene in three B. rapa strains: S-8, Osm, and the self-compatible var. Yellow Sarson (YS). Nucleotide sequence analysis of the MIP-MODgenes isolated from the three strains revealed that all three encode the same amino acid sequence and that YS and Osm contain the same MIP-MOD allele, designated MIP-MODYS. Analysis of other self-incompatible B. rapa strainsthat are homozygous for the MIP-MODYS allele indicated that high levels ofMIP-MOD transcripts are not essential for the self-incompatibility response. Furthermore, a MOD mutant generated by gamma -irradiation was found to contain a wildtype MIP-MOD gene that is expressed at normal levels. These data suggest that MIP-MOD is not MOD itself. We suggest that this gene shouldbe renamed MLM (for MIP gene linked to MOD).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:36:56