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Titolo:
Stability and inheritance of methylation states at PstI sites in Pisum
Autore:
Knox, MR; Ellis, THN;
Indirizzi:
John Innes Ctr, Norwich NR4 7UH, Norfolk, England John Innes Ctr Norwich Norfolk England NR4 7UH NR4 7UH, Norfolk, England
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 3, volume: 265, anno: 2001,
pagine: 497 - 507
SICI:
1617-4615(200105)265:3<497:SAIOMS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT LENGTH POLYMORPHISMS; GENETIC DIVERSITY ASSESSMENT; AFLP MARKERS; LINKAGE MAP; CYTOSINE METHYLATION; DNA METHYLATION; SEGREGATION DISTORTION; PACIFIC-NORTHWEST; MOLECULAR MARKERS; WHEAT CULTIVARS;
Keywords:
pea; DNA methylation; mapping; phylogeny; AFLP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Knox, MR John Innes Ctr, Coloney Lane, Norwich NR4 7UH, Norfolk, England John Innes Ctr Coloney Lane Norwich Norfolk England NR4 7UH gland
Citazione:
M.R. Knox e T.H.N. Ellis, "Stability and inheritance of methylation states at PstI sites in Pisum", MOL GENET G, 265(3), 2001, pp. 497-507

Abstract

The nuclear genome of pea is heavily methylated and the stability of this methylation pattern is unknown. In this study we investigated the stabilityof DNA methylation and its contribution to restriction fragment polymorphism as judged by AFLP fragment differences. To do this the results of a conventional AFLP analysis were compared with those obtained by a related procedure performed on pre-amplified DNA which provided a 5-methylcytosine-free DNA template (SDAFLP). Genetic mapping in a recombinant inbred (RI) population showed that polymorphisms attributable to different methylation states of PstI sites were abundant, and generally appeared to be stably inherited,although occasional failures of the inheritance of methylation states havebeen found. Assessments of genetic diversity by AFLP and SDAFLP were in general agreement with each other and with the currently accepted phylogeny of Pisum, but within cultivated groups the number of differences appeared tobe exaggerated by AFLP. The data suggest that epigenetic differences may have played a role in the domestication of pea.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:58:53