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Titolo:
Characterization and expression of beta-1,3-glucanase genes in peach
Autore:
Thimmapuram, J; Ko, TS; Korban, SS;
Indirizzi:
Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 es & Environm Sci, Urbana, IL 61801 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 3, volume: 265, anno: 2001,
pagine: 469 - 479
SICI:
1617-4615(200105)265:3<469:CAEOBG>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENESIS-RELATED PROTEINS; MESSENGER-RNAS; ACQUIRED-RESISTANCE; TOBACCO PLANTS; SALICYLIC-ACID; CHITINASE; ETHYLENE; ARABIDOPSIS; SEQUENCES; INDUCTION;
Keywords:
beta-1,3-glucanase; defense gene; ethylene; Prunus persica; Xanthomonas campestris pv. pruni elicitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Korban, SS Univ Illinois, Dept Nat Resources & Environm Sci, 310 Madigan Bldg,1201 W Gregory Dr, Urbana, IL 61801 USA Univ Illinois 310 Madigan Bldg,1201 W Gregory Dr Urbana IL USA 61801
Citazione:
J. Thimmapuram et al., "Characterization and expression of beta-1,3-glucanase genes in peach", MOL GENET G, 265(3), 2001, pp. 469-479

Abstract

beta -1,3-glucanase is one of the pathogenesis-related (PR) proteins involved in plant defense responses. A peach beta -1,3-glucanase gene, designated PpGnsl, has been isolated and characterized. The deduced amino acid sequence of the product of PpGns indicates that it is a basic isoform (pI 9.8), and contains a putative signal peptide of 38 amino acids but has no C-terminal extension. Amino acid sequence comparisons revealed that PpGnsl is 69% and 67% identical to citrus and soybean (beta -1,3-glucanases, respectively. Southern analysis of total genomic DNA also indicates that at least threegenes for beta -1,3-glucanases exist in peach, forming a small gene family. Characterization of four additional clones by PCR has identified a secondbeta -1,3-glucanase gene, PpGns2. PpGns2 has been partially sequenced, andwhen compared to PpGnsl, it shows high sequence homology, 96% and 99% nucleotide identity in the first and (partial) second exons, respectively. The deduced partial sequence of the PpGns2 product displays only two differences from PpGnsl in the signal peptide and one in the (partial) mature protein(141 amino acids). The 5'-flanking promoter regions of these two genes share 90% identity in nucleotide sequences interrupted by five major gaps (4-109 nt long). The promoter region contains various sequences similar to cis-regulatory elements present in different stress-induced plant genes. In leaves and stems of peach shoot cultures grown in vitro, PpGnsl is induced within 12 h after exposure to a culture filtrate of Xanthomonas campestris pv.pruni or ethephon. However, it is not induced following treatment with mercuric chloride.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 03:11:27