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Titolo:
Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestiuvm and Haynaldia villosa Schur
Autore:
Zhou, A; Xia, G; Zhang, X; Chen, H; Hu, H;
Indirizzi:
Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China Shandong Univ Jinan Peoples R China 250100 Jinan 250100, Peoples R China Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China Chinese AcadSci Beijing Peoples R China 100101 100101, Peoples R China
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 3, volume: 265, anno: 2001,
pagine: 387 - 393
SICI:
1617-4615(200105)265:3<387:AOCAOD>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYCOPERSICON-ESCULENTUM; AESTIVUM L; INSITU HYBRIDIZATION; PLANT-REGENERATION; SOLANUM-TUBEROSUM; JUNCEA; WHEAT;
Keywords:
Triticum aestivum; Haynaldia villosa; somatic hybrids; genomic in situ hybridization (GISH); organellar genes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Xia, G Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China ShandongUniv Jinan Peoples R China 250100 50100, Peoples R China
Citazione:
A. Zhou et al., "Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestiuvm and Haynaldia villosa Schur", MOL GENET G, 265(3), 2001, pp. 387-393

Abstract

Intergeneric somatic hybridization between wheat (cv. Jinan 177) protoplasts that have 24-28 chromosomes and Haynaldia villosa protoplasts containing11-14 chromosomes was carried out by the polyethylene glycol (PEG) method. A high frequency of hybrid calli and plants were obtained from the fusion products, as revealed by cytological and biochemical techniques and by PCR analysis of 5S rDNA spacer sequences. GISH (genomic in situ hybridization) analysis confirmed the presence of chromosomes from both parents in the hybrid clones and the common occurrence of translocations between them. The RFLP analysis of the organellar DNA using mitochondrion and chloroplast-specific probes revealed that mitochondria from both parents existed in the cells of hybrid calli and their recombination, whereas chloroplasts segregated and recombined randomly. The gross morphology of hybrid plants resembled that of wheat, but the gross morphology of their ovaries and anthers were intermediate between those of the two parents. The relationship between hybridplant regeneration and the balance of genetic materials in hybrid clones is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:45:58