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Titolo:
A scytalone dehydratase gene from Ophiostoma floccosum restores the melanization and pathogenicity phenotypes of a melanin-deficient Colletotrichum lagenarium mutant
Autore:
Wang, HL; Kim, SH; Breuil, C;
Indirizzi:
Univ British Columbia, Dept Wood Sci, Chair Forest Prod Biotechnol, Vancouver, BC V6T 1Z4, Canada Univ British Columbia Vancouver BC Canada V6T 1Z4 ver, BC V6T 1Z4, Canada
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 266, anno: 2001,
pagine: 126 - 132
SICI:
1617-4615(200109)266:1<126:ASDGFO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALTERNARIA-ALTERNATA; MAGNAPORTHE-GRISEA; BIOLISTIC TRANSFORMATION; CRYPTOCOCCUS-NEOFORMANS; STRUCTURAL-ANALYSIS; SEQUENCE DATA; GENOMIC DNA; BIOSYNTHESIS; PIGMENTATION; PENETRATION;
Keywords:
sap-staining fungi; DHN melanin; pigmentation; complementation; scytalone dehydratase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Breuil, C Univ British Columbia, Dept Wood Sci, Chair Forest Prod Biotechnol, 2424 Main Wall, Vancouver, BC V6T 1Z4, Canada Univ British Columbia 2424 Main Wall Vancouver BC Canada V6T 1Z4
Citazione:
H.L. Wang et al., "A scytalone dehydratase gene from Ophiostoma floccosum restores the melanization and pathogenicity phenotypes of a melanin-deficient Colletotrichum lagenarium mutant", MOL GENET G, 266(1), 2001, pp. 126-132

Abstract

Scytalone dehydratase is involved in the production of fungal dihydroxynaphthalene (DHN) melanin. We have isolated and characterized OSD1, a gene encoding scytalone dehydratase from the sap-staining fungus Ophiostoma floccosum by PCR-based cloning. Sequence analysis suggests that the OSD1 gene encodes a protein of 216 amino acids with a molecular weight of 24.2 kDa that shows 51-70% sequence identity to other scytalone dehydratases. The cloned OSD1 contains two introns of 76 by and 63 by in length, and is the longest scytalone dehydratase gene sequence so far reported. Transformation of a DHNmelanin-deficient. nonpathogenic, mutant of Colletotrichum lagenarium withthe OSD1 gene restored melanin production and pathogenicity. The ability of the mutant to produce the OSD1 gene product was confirmed by RT-PCR analysis. These data show that the cloned OSD1 gene product can function in the DHN melanin biosynthetic pathway in C. lagenarium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 03:10:52