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Titolo:
DNA protection mechanisms are not involved in the radioresistance of the hyper thermophilic archaea Pyrococcus abyssi and P-furiosus
Autore:
Gerard, E; Jolivet, E; Prieur, D; Forterre, P;
Indirizzi:
Univ Paris Sud, Inst Genet & Microbiol, CNRS, UMR 8621, F-91405 Orsay, France Univ Paris Sud Orsay France F-91405 NRS, UMR 8621, F-91405 Orsay, France CNRS, UMR 6539, IUEM, UBO, F-29280 Plouzane, France CNRS Plouzane FranceF-29280 R 6539, IUEM, UBO, F-29280 Plouzane, France
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 266, anno: 2001,
pagine: 72 - 78
SICI:
1617-4615(200109)266:1<72:DPMANI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IONIZING-RADIATION; DEINOCOCCUS RADIODURANS; GAMMA-IRRADIATION; STRAND BREAKS; SP-NOV; BACTERIA; PROTEINS; DAMAGE; OXYGEN; CELLS;
Keywords:
hyperthermophile; archaea; radioresistance; DNA double-stranded breaks;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Gerard, E Univ Paris Sud, Inst Genet & Microbiol, CNRS, UMR 8621, Bat 409,F-91405 Orsay, France Univ Paris Sud Bat 409 Orsay France F-91405 91405 Orsay, France
Citazione:
E. Gerard et al., "DNA protection mechanisms are not involved in the radioresistance of the hyper thermophilic archaea Pyrococcus abyssi and P-furiosus", MOL GENET G, 266(1), 2001, pp. 72-78

Abstract

Hyperthermophilic archaea of the genus Pyrococcus are resistant to gamma radiation, suggesting that efficient mechanisms for DNA repair exist in these organisms. To determine whether protective mechanisms might also be implicated in this radioresistance, we have estimated the linear density of DNA double-stranded breaks caused by gamma irradiation in the genomic DNA of two Pyrococcus species, using Escherichia coli and the radioresistant bacterium Deinococcus radiodurans as controls. The linear density of double-stranded breaks was essentially the same in all four microorganisms when irradiation was carried under similar anaerobic conditions, indicating that no specific DNA protection mechanisms exist in Pyrococcus species. Using one- and two-dimensional gel electrophoresis we compared the protein patterns from Pyrococcus abyssi and P. furiosus cells that had or had not been exposed to gamma rays. We did not detect any significant protein induction following DNA damage in either species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:35:52