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Titolo:
Cytochrome c is not essential for viability of the fungus Aspergillus nidulans
Autore:
Bradshaw, RE; Bird, DM; Brown, S; Gardiner, RE; Hirst, P;
Indirizzi:
Massey Univ, Inst Mol Biosci, Palmerston North, New Zealand Massey Univ Palmerston North New Zealand Palmerston North, New Zealand Massey Univ, Inst Fundamental Sci, Palmerston North, New Zealand Massey Univ Palmerston North New Zealand Palmerston North, New Zealand DB Breweries, Auckland, New Zealand DB Breweries Auckland New ZealandDB Breweries, Auckland, New Zealand
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 266, anno: 2001,
pagine: 48 - 55
SICI:
1617-4615(200109)266:1<48:CCINEF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROSPORA-CRASSA; GENE; MITOCHONDRIA; DEFICIENCY; DISRUPTION; MUTATIONS; SELECTION; PLASMID; CLONING; YEAST;
Keywords:
cytochrome c; Aspergillus; gene replacement; alternative respiration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Bradshaw, RE Massey Univ, Inst Mol Biosci, Private Bag 11222, Palmerston North, New Zealand Massey Univ Private Bag 11222 Palmerston North New Zealand
Citazione:
R.E. Bradshaw et al., "Cytochrome c is not essential for viability of the fungus Aspergillus nidulans", MOL GENET G, 266(1), 2001, pp. 48-55

Abstract

The filamentous fungus Aspergillus nidulans is an obligate aerobe, which is capable of anaerobic survival, but not anaerobic growth. Since cytochromec forms an essential part of the oxidative respiratory pathway it was expected that mutants lacking this component would be non-viable. Gene replacement of one homologue of the cycA (cytochrome c) gene was carried out in a diploid strain. Benomyl-induced haploidisation of this diploid yielded all cycA(+) haploid colonies, initially suggesting that loss of cycA was indeed lethal. However. use of an alternative unbiased method to recover haploids yielded viable, but slow-growing, cycA(-) mutants. Replacement of the cycA locus in the cycA(-)mutants was verified by Southern blotting. Spectral analysis confirmed the absence of detectable levels of cytochrome c, and respiratory insensitivity to cyanide suggested the absence of cytochrome c-dependent respiration. Growth parameters were consistent with those expected of a cycA(-) mutant. Compared to the wild type, the mutants grew slowly on fermentable carbon sources, did not grow on non-fermentable carbon sources, and produced higher levels of ethanol. To our knowledge, this is the first report of a filamentous fungus that remains viable after complete eliminationof a functional cytochrome c gene. We propose that the mutants are viable due to their ability to ferment and to use alternative respiratory pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:07:55