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Titolo:
Altered membrane fluidity and signal transduction in the platelets from patients of thrombotic stroke
Autore:
Srivastava, K; Dash, D;
Indirizzi:
Banaras Hindu Univ, Inst Med Sci, Dept Biochem, Varanasi 221005, Uttar Pradesh, India Banaras Hindu Univ Varanasi Uttar Pradesh India 221005 tar Pradesh, India
Titolo Testata:
MOLECULAR AND CELLULAR BIOCHEMISTRY
fascicolo: 1-2, volume: 224, anno: 2001,
pagine: 143 - 149
SICI:
0300-8177(2001)224:1-2<143:AMFAST>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE CEREBRAL-ISCHEMIA; HUMAN-BLOOD-PLATELETS; PROTEIN-PHOSPHORYLATION; PROCOAGULANT ACTIVITY; MOLECULAR MECHANISMS; ACTIVATED PLATELETS; TYROSINE KINASES; CALPAIN; AGGREGATION; CLEAVAGE;
Keywords:
anisotropy; calpain; membrane microviscosity; platelet activation; signal transduction; tyrosine phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Dash, D Banaras Hindu Univ, Inst Med Sci, Dept Biochem, Varanasi 221005, Uttar Pradesh, India Banaras Hindu Univ Varanasi Uttar Pradesh India 221005esh, India
Citazione:
K. Srivastava e D. Dash, "Altered membrane fluidity and signal transduction in the platelets from patients of thrombotic stroke", MOL C BIOCH, 224(1-2), 2001, pp. 143-149

Abstract

Several earlier studies have implicated platelet activation with the pathogenesis of thrombotic stroke. In this report we have studied the changes inmembrane physical microenvironment and signal transduction in the platelets obtained from the patients with thrombotic stroke. Aggregation induced bythe synthetic agonist thrombin receptor-activating peptide was significantly enhanced (p < 0.001) in the platelets obtained from the patients. Steady-state fluorescence anisotropy measurements using diphenylhexatriene reflected a significant increase in membrane microviscosity from 3.315 (+/- 0.103) in the control to 4.600 (+/- 0.119) in the stroke. Proteins of relative mobilities of 131, 100, 47 and 38 kDa were found to remain phosphorylated ontyrosine in the resting platelets obtained from thrombotic stroke patientswhile they were not phosphorylated in the control counterparts. Besides, calpain, a calcium dependent thiol protease present in the platelets, was found to remain active in this disease as reflected from the proteolysis of calpain substrates. Taken together, these data indicated abnormal circulating platelets in the patients of thrombotic stroke, which could contribute tothe etiopathogenesis of this disease.

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Documento generato il 28/01/20 alle ore 20:56:35