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Titolo:
Serological diagnosis of equine influenza using the hemagglutinin protein produced in a baculovirus expression system
Autore:
Sugiura, T; Sugita, S; Imagawa, H; Kanaya, T; Ishiyama, S; Saeki, N; Uchiyama, A; Tanigawa, M; Kuwano, A;
Indirizzi:
Japan Racing Assoc, Equine Res Inst, Epizoot Res Stn, Kokubunji, Tochigi 3290412, Japan Japan Racing Assoc Kokubunji Tochigi Japan 3290412 Tochigi 3290412, Japan
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 1, volume: 98, anno: 2001,
pagine: 1 - 8
SICI:
0166-0934(200110)98:1<1:SDOEIU>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSECT CELLS; VIRUS; ANTIBODIES; OUTBREAK;
Keywords:
baculovirus; equine influenza virus; hemagglutinin protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Sugiura, T Japan Racing Assoc, Equine Res Inst, Epizoot Res Stn, 1400-4 Shiba, Kokubunji, Tochigi 3290412, Japan Japan Racing Assoc 1400-4 Shiba Kokubunji Tochigi Japan 3290412
Citazione:
T. Sugiura et al., "Serological diagnosis of equine influenza using the hemagglutinin protein produced in a baculovirus expression system", J VIROL MET, 98(1), 2001, pp. 1-8

Abstract

The hemagglutinin (HA) protein of an equine influenza strain, A/equine/La Plata/1/93 (LP/93), was produced using a baculovirus expression system. Silkworm larvae inoculated with recombinant baculovirus expressed high quantities of the HA protein which was then purified to greater than 95%,, purity by fetuin-affinity chromatography. Purified HA protein was used subsequently in an ELISA for detection of antibodies in horse sera. Two hundred serum samples from vaccinated racehorses were reacted on ELISA plates coated with40.0 ng/ml of purified HA protein. Subsequent optical density (OD) levels revealed titers which correlated highly with respective hemagglutinin inhibition (HI) antibody titers which ranged from < 1:8 to 1:256 (correlation coefficient among them was 0.850). ELISA OD levels and HI titers increased at5 and 7 days post-inoculation, respectively, in a horse inoculated intranasally with LP/93. Respective antibody levels were observed to change in an essentially parallel manner during a period of I month. Similarly, ELISA ODlevels correlated with HI titers in horses during a period of 6 weeks following intramuscular inoculation with inactivated single-strain vaccines containing LP/93, A/equine/Kentucky/1/81 (H3N8) or A/equine/Rome/5/91 (H3N8). A similar pattern was also observed in eight horses throughout a 10-week period following inoculation with a commercially available inactivated trivalent vaccine containing A/equine/Newmarket/1/77(H7N7), A/equine/Kentucky/81 and LP/93. From these results, it is suggested that this ELISA system couldbe used for disease diagnosis and surveillance of HI antibody titers amongvaccinated horses. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 11:29:51