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Titolo:
Protein transfection of intact microvessels specifically modulates vasoreactivity and permeability
Autore:
Tinsley, JH; Zawieja, DC; Wu, MH; Ustinova, EE; Xu, WJ; Yuan, SY;
Indirizzi:
Texas A&M Univ, Hlth Sci Ctr, Cardiovasc Res Inst, Dept Med Physiol, Temple, TX 76504 USA Texas A&M Univ Temple TX USA 76504 Dept Med Physiol, Temple, TX 76504 USA Texas A&M Univ, Hlth Sci Ctr, Cardiovasc Res Inst, Dept Surg, Temple, TX 76504 USA Texas A&M Univ Temple TX USA 76504 Inst, Dept Surg, Temple, TX 76504 USA
Titolo Testata:
JOURNAL OF VASCULAR RESEARCH
fascicolo: 5, volume: 38, anno: 2001,
pagine: 444 - 452
SICI:
1018-1172(200109/10)38:5<444:PTOIMS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE-C; NITRIC-OXIDE; ENDOTHELIAL PERMEABILITY; VENULAR PERMEABILITY; GUANYLATE-CYCLASE; HYPERPERMEABILITY; ARTERIOLES; ACTIVATION; INCREASES; MECHANISM;
Keywords:
permeability; transfection; PKC; nitric oxide synthase; vasodilation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Tinsley, JH Texas A&M Univ, Hlth Sci Ctr, Cardiovasc Res Inst, Dept Med Physiol, 702 SW HK Dodgen Loop,Room 206F, Temple, TX 76504 USA Texas A&M Univ702 SW HK Dodgen Loop,Room 206F Temple TX USA 76504
Citazione:
J.H. Tinsley et al., "Protein transfection of intact microvessels specifically modulates vasoreactivity and permeability", J VASC RES, 38(5), 2001, pp. 444-452

Abstract

Precise regulation of microvascular tone and barrier function is essentialfor proper coronary perfusion and performance. Agonist-induced alterationsin either or both of these functions ultimately lead to microcirculatory dysfunction and cardiac insufficiency. Two important pathways involved in regulating vasomotor response and barrier function are the: activation of nitric oxide synthase (BIOS) and upregulation of protein kinase C (PKC). To date, studies of these two signaling proteins have relied mainly on pharmacological approaches. Unfortunately, the specificity of various inhibitors canbe cause for concern. In order to address this problem, a protein transfection technique we developed for cultured endothelial cells has been modified and applied to isolated, intact coronary microvessels. Our results from green fluorescent protein transfection in arterioles and venules showed thatthis procedure could be used to introduce proteins into the microvascular wall. By transfecting inhibitor peptides against NOS and PKC into coronary arterioles and venules, we have been able to determine the specific roles of these two, enzymes in vasodilation and hyperpermeability responses. Copyright (C) 2001 S. Karger AG, Baser.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 02:00:12