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Titolo:
Chiroptical, ESMS and NMR spectroscopic study of the interaction of enantiopure lanthanide complexes with selected self-complementary dodecamer oligonucleotides
Autore:
Bobba, G; Dickins, RS; Kean, SD; Mathieu, CE; Parker, D; Peacock, RD; Siligardi, G; Smith, MJ; Williams, JAG; Geraldes, CFGC;
Indirizzi:
Univ Durham, Dept Chem, Durham DH1 3LE, England Univ Durham Durham England DH1 3LE m, Dept Chem, Durham DH1 3LE, England Univ Glasgow, Dept Chem, Glasgow G12 8QQ, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G12 8QQ ow G12 8QQ, Lanark, Scotland Univ London Kings Coll, Dept Pharm, London SE7 8WA, England Univ London Kings Coll London England SE7 8WA m, London SE7 8WA, England Univ Coimbra, Ctr Neurosci, Dept Biochem, P-3000 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3000 t Biochem, P-3000 Coimbra, Portugal
Titolo Testata:
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
fascicolo: 9, , anno: 2001,
pagine: 1729 - 1737
SICI:
1472-779X(200109):9<1729:CEANSS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CIRCULARLY-POLARIZED LUMINESCENCE; TIME-RESOLVED FLUORESCENCE; MASS-SPECTROMETRY; DNA-BINDING; CONFORMATIONAL TRANSITIONS; SUPRAMOLECULAR CHEMISTRY; TETRAAMIDE COMPLEXES; HOMOGENEOUS SOLUTION; EUROPIUM CHELATE; MINOR-GROOVE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Parker, D Univ Durham, Dept Chem, South Rd, Durham DH1 3LE, England Univ Durham South Rd Durham England DH1 3LE am DH1 3LE, England
Citazione:
G. Bobba et al., "Chiroptical, ESMS and NMR spectroscopic study of the interaction of enantiopure lanthanide complexes with selected self-complementary dodecamer oligonucleotides", J CHEM S P2, (9), 2001, pp. 1729-1737

Abstract

The interaction of Delta- and Lambda -Eu and Yb cationic complexes bearingan N-methylphenanthridinium chromophore with [(CG)(6)](2), [(AT)(6)], and [CGCGAATTCGCG](2) has been interrogated by ESMS, H-1 NMR, absorption, difference circular dichroism, fluorescence quenching and Ln luminescence emission spectroscopy. Stepwise complexation occurs with up to 3:1 limiting stoichiometry for [(CG)(6)](2) and 2:1 for [CGCGAATTCGCG](2), as indicated by absorption measurements and direct ESMS observation of the non-covalent duplex adducts. Binding to [(CG),], occurred with an affinity of 8.7 x 10(6) M-1duplex(-1) for the Delta -Eu isomer which was 50 times greater than to [(AT)(6)](2). A primary component of the free energy of binding has been linked to an intercalative interaction, supported by absorption, ICD and fluorescence quenching characteristics: The lanthanide coordination. environment and local helicity remain unchanged but the oligonucleotide underwent distinctive changes in local helicity and pitch which were sensitive to the handedness of the Ln complex and in certain cases to the nature of the Ln ion (Yb vs. Eu). With [CGCGAATTCGCG](2) H-1 NMR TOCSY and NOESY analysis in the presence of increasing concentrations of the Gd analogues revealed that the most avid complex binding site was located in the centre of the oligonucleotide, with the Delta -isomer binding more strongly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:42:34