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Titolo:
Effects of the heterogeneous nuclear ribonucleoprotein U (hnRNP U/SAF-A) on glucocorticoid-dependent transcription in vivo
Autore:
Eggert, H; Schulz, M; Fackelmayer, FO; Renkawitz, R; Eggert, M;
Indirizzi:
Univ Giessen, Genet Inst, D-35392 Giessen, Germany Univ Giessen Giessen Germany D-35392 enet Inst, D-35392 Giessen, Germany Heinrich Pette Inst Expt Virol & Immunol, Dept Mol Cell Biol, D-20251 Hamburg, Germany Heinrich Pette Inst Expt Virol & Immunol Hamburg Germany D-20251 Germany
Titolo Testata:
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 78, anno: 2001,
pagine: 59 - 65
SICI:
0960-0760(200107)78:1<59:EOTHNR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING; RECEPTOR SUPERFAMILY; ENHANCER ELEMENTS; MATRIX PROTEIN; HELA-CELLS; SAF-A; DOMAINS; RNA; SITES; GENE;
Keywords:
glucocorticoid receptor; hnRNP U/SAF-A; nuclear matrix;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Eggert, M Univ Giessen, Genet Inst, Heinrich Buff Ring 58-62, D-35392 Giessen, Germany Univ Giessen Heinrich Buff Ring 58-62 Giessen Germany D-35392y
Citazione:
H. Eggert et al., "Effects of the heterogeneous nuclear ribonucleoprotein U (hnRNP U/SAF-A) on glucocorticoid-dependent transcription in vivo", J STEROID B, 78(1), 2001, pp. 59-65

Abstract

The glucocorticoid receptor (GR) is a ligand dependent transcription factor, which regulates the transcription of multiple hormone-dependent genes. The transcriptional regulation by GR takes place by interaction of GR with the basal transcription machinery and by recruiting glucocorticoid receptor interacting proteins (GRIPs). Previously we identified hnRNP U/SAF-A as a factor interfering with GR-dependent transcription by repressing glucocorticoid induced activation. To gain insight into the mechanisms that govern this interference, we have now investigated the transcription of GR-dependent reporter genes in Ltk-cells transiently transfected with a variety of hnRNPU constructs. We demonstrate that a hnRNP U construct lacking the GR-binding domain acts as a dominant negative factor that now enhances GR-driven transcription. In addition, hnRNP U repression of glucocorticoid induced transcription was found to be dependent on the amount of cotransfected GR, where a high amount of GR leads to ligand-inducible repression of GR-dependent reporter gene activity by hnRNP U, whereas low amounts of GR showed nearly no effect. The relative concentrations of GR, hnRNP U and DNA-binding sitesfor GR are important for the effect of hnRNP U on transcription, suggesting a model where hnRNP-U acts as a storage site for intranuclear GR. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 13:08:28