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Titolo:
Isolation and amino acid sequence of two new PR-4 proteins from wheat
Autore:
Caruso, C; Nobile, M; Leonardi, L; Bertini, L; Buonocore, V; Caporale, C;
Indirizzi:
Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy UnivTuscia Viterbo Italy I-01100 iol & Agrochim, I-01100 Viterbo, Italy
Titolo Testata:
JOURNAL OF PROTEIN CHEMISTRY
fascicolo: 4, volume: 20, anno: 2001,
pagine: 327 - 335
SICI:
0277-8033(200105)20:4<327:IAAASO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENESIS-RELATED PROTEINS; LIMITED PROTEOLYTIC SITES; CHITIN-BINDING PROTEINS; BARLEY SEED; HOMOLOGOUS PROTEINS; ANTIFUNGAL ACTIVITY; PLANT CHITINASES; HEVEIN; BARWIN; PURIFICATION;
Keywords:
amino acid sequence; molecular modeling; pathogenesis-related proteins; wheat kernel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Caruso, C Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy Univ Tuscia Viterbo Italy I-01100 chim, I-01100 Viterbo, Italy
Citazione:
C. Caruso et al., "Isolation and amino acid sequence of two new PR-4 proteins from wheat", J PROTEIN C, 20(4), 2001, pp. 327-335

Abstract

We have purified and characterized two new pathogenesis-related (PR) proteins from wheat belonging to the PR-4 family. We named the proteins wheatwin3 and wheatwin4 in analogy with the previously characterized wheatwin1 and wheatwin2. Their isoelectric points were 7.1 and 8.4, respectively, We determined the complete amino acid sequence of both proteins by a rapid approach based on the knowledge of the primary structures of the homologous wheatwin1 and wheatwin2. Wheatwin3 differs from wheatwin1 in one substitution at position 88, while wheatwin4 differs from wheatwin2 in one substitution at position 78. The secondary structure and solvent accessibility of these residues were determined on the three-dimensional model of wheatwin1. Residue 88 was very accessible and was located in a flexible region. Preliminary results indicate that, like wheatwin1 and wheatwin2, wheatwin3 and wheatwin4 have antifungal activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 09:04:54