Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Fructan biosynthesis and fructosyltransferase evolution: Expression of the6-SFT (sucrose : fructan 6-fructosyltransferase) gene in crested wheatgrass (Agropyron cristatum)
Autore:
Wei, JZ; Chatterton, NJ;
Indirizzi:
Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA Utah State Univ Logan UT USA 84322 e & Range Res Lab, Logan, UT 84322 USA
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 9, volume: 158, anno: 2001,
pagine: 1203 - 1213
SICI:
0176-1617(200109)158:9<1203:FBAFEE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
BARLEY LEAVES; KEY ENZYME; EXCISED LEAVES; CLONING; INVERTASES; CARBOHYDRATE; METABOLISM; OLIGOMERS; TIMOTHY; TOBACCO;
Keywords:
Agropyron; fructan; fructosyltransferase; gene-expression; invertase; phylogeny; sucrose : sucrose 6-fructosyltransferase; 6-SFT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Chatterton, NJ Univ Calif San Diego, Div Biol, La Jolla, CA 92093 USA UnivCalif San Diego La Jolla CA USA 92093 a, CA 92093 USA
Citazione:
J.Z. Wei e N.J. Chatterton, "Fructan biosynthesis and fructosyltransferase evolution: Expression of the6-SFT (sucrose : fructan 6-fructosyltransferase) gene in crested wheatgrass (Agropyron cristatum)", J PLANT PHY, 158(9), 2001, pp. 1203-1213

Abstract

Crested wheatgrass (Agropyron cristatum), an important cool-season grass, contains a complexity of fructans, Including levans, inulins and graminans. Fructan types are characterized by unique combinations of carbon-carbon linkages. This study established the DNA sequence for the 6-SFT gene (sucrose: fructan 6-fructosyltransferase) in A. cristatum and compared the sequencewith invertases and fructosyltransferases from various species. Detachmentwith Illumination, as well as cool treatment of crested wheatgrass leaves induced the accumulation of fructans. All three types of fructans, beta -2,1; beta -2,6 and graminans (combination of both beta -2,1 and beta -2,6 linkages) were detected. The cDNA of 6-SFT was cloned from A. cristatum. The deduced amino acid sequences showed 85 % identity with barley (Hordeum vulgare) 6-SFT. It also exhibited high homology with other fructosyltransierasesand Invertases. The expression of 6-SFT mRNA was analyzed with Northern hybridization. mRNA of the 6-SFT gene accumulated after 8 h of Illumination In detached leaves and remained at a steady state for 36 hours. Under low temperature (10 degreesC/5 degreesC, day/night), the mRNA of 6-SFT began to accumulate after 8 h and reached the highest level at 2 d and then declined after 8 days of treatment. A coincidence of fructan and 6-SFT mRNA accumulation In crested wheatgrass suggests that 6-SFT has a role in fructan biosynthesis. A cladogram comparison of the amino acid sequences of most known sequences of fructosyltransferases and 23 invertases shows separations into various clades of like genes. 6-SFT does not contain the unique NDPNG amino acid sequence characteristic of invertases. Sequence analysis Indicated that fructosyltransferases likely evolved separately in dicot and monocot species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:40:36