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Titolo:
Molecular cloning and characterization of salt responsive genes in rice (Oryza sativa)
Autore:
Pillai, MA; Yanagihara, S; Akiyama, T;
Indirizzi:
Hokkaido Natl Agr Expt Stn, Low Temp Physiol Lab, Sapporo, Hokkaido 062, Japan Hokkaido Natl Agr Expt Stn Sapporo Hokkaido Japan 062 Hokkaido 062, Japan Japan Int Res Ctr Agr Sci, Okinawa Subtrop Stn, Okinawa 9070002, Japan Japan Int Res Ctr Agr Sci Okinawa Japan 9070002 , Okinawa 9070002, Japan
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 9, volume: 158, anno: 2001,
pagine: 1189 - 1194
SICI:
0176-1617(200109)158:9<1189:MCACOS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
OSMOTIC-STRESS; ABSCISIC-ACID; CYSTEINE PROTEINASES; EXPRESSION; TEMPERATURE; TOLERANCE; TOMATO; GERMINATION; SUBUNIT; LIGHT;
Keywords:
cDNA; differential screening; Northern hybridization; Oryza sativa L.; salt; Southern hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Pillai, MA Hokkaido Natl Agr Expt Stn, Low Temp Physiol Lab, Sapporo, Hokkaido 062, Japan Hokkaido Natl Agr Expt Stn Sapporo Hokkaido Japan 062 2, Japan
Citazione:
M.A. Pillai et al., "Molecular cloning and characterization of salt responsive genes in rice (Oryza sativa)", J PLANT PHY, 158(9), 2001, pp. 1189-1194

Abstract

Differential screening was performed on a cDNA library made from RNAs isolated from rice seedlings (variety TSA2), grown in water culture with 3 h osmotic shock (150mmol/L NaCl). Sixteen cDNA clones showing preferential hybridization to RNA from salt shocked seedlings were isolated. Three clones encoding salt responsive protein were selected by Northern hybridization study, One of the clones OSSRI represented a gene that is expressed at high levels during 24 In NaCl treatment. The DNA sequence analysis of OSSRI indicated a similarity to the deduced amino acid sequence of inositol phosphate kinase like protein of Arabidopsis thaliana. Another clone OSSRII showed Identity to the photosystem II reaction center W protein of Arabidopsis thaliana. The clone OSSRIII was highly similar to cysteine proteinase inhibitor which is strongly associated with a chilling inducible and drought tolerant protein. Hybridization of the clone OSSRI to genomic DNA digested with EcoR1, EcoRV, HindIII, and Sal1 indicated that a single copy of the gene encodedthe salt inducible transcript. RNA blot hybridization showed that mRNA of 1.3, 1.0, 0.9kb for the clones OSSRI, OSSRII and OSSRIII, respectively, accumulated over time up to 24 h.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:26:19