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Titolo:
Performance of a commercial immunoassay for detection and differentiation of periodontal marker bacteria: Analysis of immunochemical performance withclinical samples
Autore:
Eggert, FM; McLeod, MH; Flowerdew, G;
Indirizzi:
Univ Alberta, Dept Dent, Fac Med & Dent, Edmonton, AB T6G 2N8, Canada UnivAlberta Edmonton AB Canada T6G 2N8 ent, Edmonton, AB T6G 2N8, Canada Dalhousie Univ, Dept Community Hlth & Epidemiol, Halifax, NS, Canada Dalhousie Univ Halifax NS Canada y Hlth & Epidemiol, Halifax, NS, Canada
Titolo Testata:
JOURNAL OF PERIODONTOLOGY
fascicolo: 9, volume: 72, anno: 2001,
pagine: 1201 - 1209
SICI:
0022-3492(200109)72:9<1201:POACIF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED IMMUNOSORBENT-ASSAY; ALVEOLAR BONE LOSS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; PORPHYROMONAS-GINGIVALIS; PREVOTELLA-INTERMEDIA; MONOCLONAL-ANTIBODIES; ADULT PERIODONTITIS; SUBGINGIVAL PLAQUE; REFRACTORY PERIODONTITIS; BACTEROIDES-GINGIVALIS;
Keywords:
comparison studies; immunoassay, enzyme; periodontitis/diagnosis; periodontitis/microbiology; dental plaque/diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Eggert, FM Univ Alberta, Dept Dent, Fac Med & Dent, Edmonton, AB T6G 2N8, Canada Univ Alberta Edmonton AB Canada T6G 2N8 on, AB T6G 2N8, Canada
Citazione:
F.M. Eggert et al., "Performance of a commercial immunoassay for detection and differentiation of periodontal marker bacteria: Analysis of immunochemical performance withclinical samples", J PERIODONT, 72(9), 2001, pp. 1201-1209

Abstract

Background: We employed a commercial immunoassay for simultaneous detection and differentiation of marker bacteria Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia and reassessed the immunochemical performance of the assay. Methods: We compared the analytical performance of the immunoassay in our study of clinical samples from 249 periodontal patients in 2 private periodontal practices with the previously reported analytical performance of the same immunoassay. We also compared immunoassay measurements of the marker bacteria in clinical samples with values obtained in other studies by directculture of the same organisms. Results: The assay produced 3 times more high-end readings than reported previously. We also reassessed and revised previously published calibration curves for the immunoassay. The immunoassay provided measurements of the marker bacteria in clinical samples from our patients that were comparable toand consistent with measurements of the same bacteria by direct culture inother studies. Conclusions: We ascribe the increased sensitivity of the immunoassay in our study to: 1) a more standardized and vigorous sample dispersion that improves release of particulate and soluble antigens from dental plaque biofilm, and 2) better visualization of the reaction product of the enzyme-linked immunoassay. High-technology assays, such as diagnostic immunoassays, have a significant potential for future development in dental diagnosis, becausethey simplify detection and measurement of biologically important markers such as specific bacteria in clinical samples. Commercial assays also have an important potential for standardization of clinical measurements of biological markers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 21:54:58