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Titolo:
Immunological evaluation of Escherichia coli-derived hepatitis C virus second envelope protein (E2) variants
Autore:
Duenas-Carrera, S; Vina, A; Garay, HE; Reyes, O; Alvarez-Lajonchere, L; Guerra, I; Gonzalez, LJ; Morales, J;
Indirizzi:
Ctr Ingn Genet & Biotecnol, HCV Dept, Vaccine Div, Havana, Cuba Ctr Ingn Genet & Biotecnol Havana Cuba Dept, Vaccine Div, Havana, Cuba
Titolo Testata:
JOURNAL OF PEPTIDE RESEARCH
fascicolo: 3, volume: 58, anno: 2001,
pagine: 221 - 228
SICI:
1397-002X(200109)58:3<221:IEOECH>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMORAL IMMUNE-RESPONSE; HYPERVARIABLE REGION-1; STRUCTURAL PROTEINS; INSECT CELLS; ANTIBODIES; CHIMPANZEES; INFECTION; PEPTIDES; PURIFICATION; IMMUNIZATION;
Keywords:
anti-HCV; E2 protein; Escherichia coli; immune recognition; synthetic peptides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Duenas-Carrera, S Ctr Ingn Genet & Biotecnol, HCV Dept, Vaccine Div, POB 6162, Havana, Cuba Ctr Ingn Genet & Biotecnol POB 6162 Havana Cuba , Cuba
Citazione:
S. Duenas-Carrera et al., "Immunological evaluation of Escherichia coli-derived hepatitis C virus second envelope protein (E2) variants", J PEPT RES, 58(3), 2001, pp. 221-228

Abstract

Two variants of the hepatitis C virus (HCV) E2 envelope protein, lacking the C-terminal domain and comprising amino acids 458-650 (E2A) and 382-605 (E2C), respectively, were efficiently produced in BL21 (DE3) Escherichia coli cells. E2A and E2C were used to immunize mice. The E2C variant induced the maximal mean antibody titer. Anti-E2C mouse sera reacted mainly with E2 synthetic peptides covering the 70 amino acid N-terminal region of the E2 protein. Moreover, a panel of anti-HCV positive human sera recognized only the E2C protein (28.2%) and the synthetic peptides covering the HVR-1 of the E2 protein (23.1%). These data indicate the existence of an immunologicallyrelevant region in the HVR-1 of the HCV E2 protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 14:53:05