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Titolo:
Tau and HMW tau phosphorylation and compartmentalization in apoptotic neuronal PC12 cells
Autore:
Shelton, SB; Johnson, GVW;
Indirizzi:
Univ Alabama, Dept Psychiat & Behav Neurobiol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Neurobiol, Birmingham, AL 35294 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 2, volume: 66, anno: 2001,
pagine: 203 - 213
SICI:
0360-4012(20011015)66:2<203:TAHTPA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-WEIGHT TAU; PERIPHERAL NERVOUS-SYSTEM; ALZHEIMERS-DISEASE; PROTEIN-PHOSPHORYLATION; PLASMA-MEMBRANE; MICROTUBULE-BINDING; SYMPATHETIC NEURONS; PARKINSONS-DISEASE; EXECUTION PHASE; DOWN-REGULATION;
Keywords:
Alzheimer disease; microtubule; cytoskeleton; caspase-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, GVW Univ Alabama, Dept Psychiat & Behav Neurobiol, Sparks Ctr Rm 1061C, Birmingham, AL 35294 USA Univ Alabama Sparks Ctr Rm 1061C BirminghamAL USA 35294 USA
Citazione:
S.B. Shelton e G.V.W. Johnson, "Tau and HMW tau phosphorylation and compartmentalization in apoptotic neuronal PC12 cells", J NEUROSC R, 66(2), 2001, pp. 203-213

Abstract

In the Alzheimer disease brain, the microtubule-associated protein tau is hyperphosphorylated. There is also evidence that apoptotic-like processes may contribute to the neuronal loss in AD. In an apoptotic model that involves replating neuronal PC12 cells without serum and nerve growth factor (NGF), tau was hyperphosphorylated. During replating, however, neurites are removed. Here, differentiated cells were maintained in serum-free media beforegrowth factor removal, thus maintaining neuritic processes during the apoptotic process and allowing for evaluation of neuritic changes. Tau phosphorylation, evaluated by immunoblotting and immunocytochemistry, was compared with various measures of cell death. Compared with control, NGF-deprived cells exhibited gradual and consistent increases of lactate dehydrogenase release over a 5-day period and a peak of caspase-3 activity at Day 2 after NGF removal. Nuclear staining demonstrated chromatin condensation in NGF-deprived cells. Apoptotic cells had thickened, tortuous, and shortened neuriticprocesses compared with control cells. Immunoblotting showed an increase in both tau and high molecular weight (HMW) tau phosphorylation during the apoptotic process. Immunoreactivity of both tau isoforms shifted from the detergent insoluble cytoskeleton to the detergent soluble compartment in the apoptotic cells. The microtubule binding of both tau isoforms from apoptotic cells also was impaired. Immunoblotting of purified plasma membrane showed preferential association of HMW tau with the plasma membrane during apoptosis. Also, plasma membrane-associated HMW tau was more phosphorylated during apoptosis. Immunocytochemistry demonstrated increased tau phosphorylation in most apoptotic cells, especially in the neurites. Tau was, however, dephosphorylated cells in the last stages of apoptosis. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:15:20