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Titolo:
Identification of nuclei associated proteins by 2D-gel electrophoresis andmass spectrometry
Autore:
Bergquist, J; Gobom, J; Blomberg, A; Roepstorff, P; Ekman, R;
Indirizzi:
Univ Gothenburg, Sahlgrens Univ Hosp, Inst Clin Neurosci, Neurochem Sect, Molndal, Sweden Univ Gothenburg Molndal Sweden eurosci, Neurochem Sect, Molndal, Sweden Uppsala Univ, Dept Analyt Chem, Inst Chem, SE-75121 Uppsala, Sweden Uppsala Univ Uppsala Sweden SE-75121 Inst Chem, SE-75121 Uppsala, Sweden Gothenburg Univ, Lundberg Lab, Dept Cell & Mol Biol Microbiol, S-41124 Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden S-41124 ol, S-41124 Gothenburg, Sweden Odense Univ, Dept Mol Biol, Odense M, Denmark Odense Univ Odense DenmarkM ense Univ, Dept Mol Biol, Odense M, Denmark
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1, volume: 109, anno: 2001,
pagine: 3 - 11
SICI:
0165-0270(20010815)109:1<3:IONAPB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
2-DIMENSIONAL GEL-ELECTROPHORESIS; POLYACRYLAMIDE GELS; BINDING-PROTEIN; CELL-NUCLEUS; LYMPHOCYTES; TRANSPORT; MATRIX; ACTIN; RNA; PROLIFERATION;
Keywords:
two-dimensional polyacrylamide gel electrophoresis; mass spectrometry; proteomics; human lymphocyte; nuclear proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Bergquist, J Univ Gothenburg, Sahlgrens Univ Hosp, Inst Clin Neurosci, Neurochem Sect, Molndal, Sweden Univ Gothenburg Molndal Sweden ochem Sect, Molndal, Sweden
Citazione:
J. Bergquist et al., "Identification of nuclei associated proteins by 2D-gel electrophoresis andmass spectrometry", J NEUROSC M, 109(1), 2001, pp. 3-11

Abstract

In clinical neuroscience as well as in many other clinical disciplines, the completion of the human genome project offers a new possibility to identify and localize the products of the genes, the proteins. Nuclear proteins are synthesized in the cytoplasm and imported into the nucleus by multiple pathways. The mechanisms by which nuclear accumulation of different molecular species occur are unclear but it is apparent that changes in the cellularand molecular events associated with the accumulation of nuclear proteins sometimes precedes transformation of cells into diseased states. The significance of the accumulation and the operation of nuclear proteins remain to be elucidated in detail. Such knowledge will play a key role in the understanding of the regulation of transcription and its disturbances in several of our most devastating diseases. In this paper we present a strategy to identify nuclear associated proteins in small samples by using two-dimensionalelectrophoresis and mass spectrometry. We have used human blood lymphocytes as a model, but the method should be rather general for any kind of tissue. Twenty two proteins were randomly chosen, and of these 18 proteins were identified by database searching of mass spectrometric data, obtained from in-gel tryptic digests of the spots. Thirteen proteins recently described with nuclear localization and function were identified, and five proteins; calgranulin B, glyceraldehyde-3-phosphate dehydrogenase (G3P2), a TATA-binding protein (ATBP), tubulin beta chain and moesin were also identified as being nuclear associated. The presented data clearly shows of the great role of two-dimensional gel electrophoresis and modern mass spectrometry in the excavation of the protein patterns on the subcellular level, and the ability to use small samples well suited for clinical screening. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 29/11/20 alle ore 06:38:53