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Titolo:
Interleukin-11 induces osteoblast differentiation and acts synergisticallywith bone morphogenetic protein-2 in C3H10T/12 cells
Autore:
Suga, K; Saitoh, M; Fukushima, S; Takahashi, K; Nara, H; Yasuda, S; Miyata, K;
Indirizzi:
Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Pharmacol Labs, Tsukuba, Ibaraki 3058585, Japan Yamanouchi Pharmaceut Co Ltd Tsukuba Ibaraki Japan 3058585 3058585, Japan Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Mol Med Labs, Tsukuba, Ibaraki 3058585, Japan Yamanouchi Pharmaceut Co Ltd Tsukuba Ibaraki Japan 3058585 3058585, Japan
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 9, volume: 21, anno: 2001,
pagine: 695 - 707
SICI:
1079-9907(200109)21:9<695:IIODAA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT HUMAN INTERLEUKIN-11; MOLECULAR-CLONING; MESSENGER-RNA; INHIBITORY FACTOR; PROGENITOR CELLS; EXPRESSION; INVITRO; MURINE; GENE; CYTOKINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Suga, K Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Pharmacol Labs, 21 Miyukigaoka, Tsukuba, Ibaraki 3058585, Japan Yamanouchi Pharmaceut Co Ltd 21 Miyukigaoka Tsukuba Ibaraki Japan 3058585
Citazione:
K. Suga et al., "Interleukin-11 induces osteoblast differentiation and acts synergisticallywith bone morphogenetic protein-2 in C3H10T/12 cells", J INTERF CY, 21(9), 2001, pp. 695-707

Abstract

Interleukin-11 (IL-11) is a pleiotropic cytokine that supports various types of hematopoietic cell growth and is involved in bone resorption. We report here the involvement of recombinant human IL-11 (rHuIL-11) in osteoblastdifferentiation in mouse mesenchymal progenitor cells, C3H10T1/2. rHuIL-11alone increased alkaline phosphatase (ALP) activity and upregulated expression levels of osteocalcin (OC), bone sialo protein (BSP), and parathyroid hormone receptor (PTHR) mRNA. rHuIL-11 had no effect on expression of type II collagen, peroxisome proliferator-activated receptor-gamma2 (PPAR-gamma2), adipocyte fatty acid-binding protein P2 (aP2), and myogenic MyoD protein(MyoD). Recombinant human bone morphogenetic protein (rHuBMP)-2 increased ALP activity and mRNA expression of these genes except for MyoD. The expression patterns of ALP activity and osteoblast-specific or chondrocyte-specific genes suggest that rHuIL-11 may be involved in early differentiation of osteoblasts at a step earlier than that which is affected by rHuBMP-2. In support of this hypothesis, combined treatment with rHuIL-11 and rHuBMP-2 synergistically increased ALP activity and mRNA expression of OC and type II collagen, rHuIL-11 also abrogated the increased levels of PPAR-gamma2, aP2 mRNA caused by rHuBMP-2. Our results suggest that rHuIL-11 alone and in combination with rHuBMP-2 can induce osteoblastic differentiation of progenitor cells and plays an important role in osteogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:08:44