Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The Ccz1 protein interacts with Ypt7 GTPase during fusion of multiple transport intermediates with the vacuole in S-cerevisiae
Autore:
Kucharczyk, R; Kierzek, AM; Slonimski, PP; Rytka, J;
Indirizzi:
Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland Polish Acad Sci Warsaw Poland PL-02106 Biophys, PL-02106 Warsaw, Poland CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 t Mol, F-91198 Gif Sur Yvette, France
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 17, volume: 114, anno: 2001,
pagine: 3137 - 3145
SICI:
0021-9533(200109)114:17<3137:TCPIWY>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUANINE-NUCLEOTIDE EXCHANGE; ELONGATION-FACTOR-TU; YEAST VACUOLE; SACCHAROMYCES-CEREVISIAE; ACTIVATING PROTEINS; GDP/GTP EXCHANGE; COMPLEX; DELIVERY; GOLGI; IDENTIFICATION;
Keywords:
Saccharomyces cerevisiae; vesicular transport; vacuole; Ypt7p;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Rytka, J Polish Acad Sci, Inst Biochem & Biophys, Pawinskiego 5A, PL-02106Warsaw, Poland Polish Acad Sci Pawinskiego 5A Warsaw Poland PL-02106 aw, Poland
Citazione:
R. Kucharczyk et al., "The Ccz1 protein interacts with Ypt7 GTPase during fusion of multiple transport intermediates with the vacuole in S-cerevisiae", J CELL SCI, 114(17), 2001, pp. 3137-3145

Abstract

Previously we have shown that the Saccharomyces cerevisiae CCZ1 (YBR131w) gene encodes a protein involved in protein trafficking. Deletion of this gene leads to fragmentation of the vacuole typical of the class B vps mutants. Genetic and biochemical data indicated that Ccz1p is required for fusion of various transport intermediates with the vacuole. Here we present data indicating that CCZ1 is a close partner of the YPT7 gene, which encodes Rab GTPase and is required for fusion of transport vesicles to vacuole and homotypic vacuole fusion. We isolated extragenic suppressors of CCZ1 deletion. All these suppressors belong to one complementation group and correspond tomutated alleles of the YPT7 gene. The mutated residues are located in two Ypt7p domains responsible for guanine binding. These data suggest that Ccz1p and Ypt7p interact physically. Coimmunoprecipitation experiments provide direct evidence that this indeed is the case. A possible mechanism of Ccz1paction is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:53:39