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Titolo:
Isolation and characterization of a novel cDNA, UBAP1, derived from the tumor suppressor locus in human chromosome 9p21-22
Autore:
Qian, J; Yang, JB; Zhang, XB; Zhang, BC; Wang, JR; Zhou, M; Tang, K; Li, WF; Zeng, ZY; Zhao, XR; Shen, SR; Li, GY;
Indirizzi:
Hunan Med Univ, Canc Res Inst, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 a 410078, Peoples R China Hunan Med Univ, Dept Gastroenterol, Xiang Ya Hosp, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 a 410078, Peoples R China
Titolo Testata:
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
fascicolo: 10, volume: 127, anno: 2001,
pagine: 613 - 618
SICI:
0171-5216(200110)127:10<613:IACOAN>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; PRIMARY NASOPHARYNGEAL CARCINOMA; DROSOPHILA-MELANOGASTER; GENE; CANCER; UBIQUITINATION; EXPRESSION; DELETIONS; FREQUENCY; SEQUENCE;
Keywords:
nasopharyngeal carcinoma; cloning and expression; UBA domain; chromosome 9p2l-22;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Li, GY Hunan Med Univ, Canc Res Inst, 88 Xiangya Rd, Changsha 410078, Peoples R China Hunan Med Univ 88 Xiangya Rd Changsha Peoples R China 410078 China
Citazione:
J. Qian et al., "Isolation and characterization of a novel cDNA, UBAP1, derived from the tumor suppressor locus in human chromosome 9p21-22", J CANC RES, 127(10), 2001, pp. 613-618

Abstract

Purpose: To clone the putative tumor suppressor gene(s) in a refined region at 9p21-22 undergoing loss of heterozygosity in nasopharyngeal carcinoma (NPC). Methods: We systematically screened the expression patterns of 25 novel ESTs (expressed sequence tags) in a minimal common deleted region of 9p21-22 in NPC. One of these ESTs was found down-regulated in NPC. Subsequently, the corresponding gene sequence of this EST was established by cDNA cloning and RACE (rapid amplification of cDNA end) procedures. Furthermore, a mouse homologue of this gene was identified. The expression of this gene was examined using Northern blot or reverse transcription-polymerase chain reaction (RT-PCR) in various human and mouse tissues. A limited screen for mutation of coding sequence of this novel human gene was undertaken using RT-PCR and direct sequencing analysis. Results: A novel gene was cloned. This gene is a new member of the UBA domain family, so we named it UBAP1 for ubiquitin-associated protein I (HUGO Gene Nomenclature Committee-approved symbol). Northern blot and RT-PCR analysis demonstrate a ubiquitous pattern of gene expression in human and mouse tissues. The direct sequencing analysis of the coding region of hU BAP1 following RT-PCR failed to reveal any mutations in a preliminary screen of NPC cell line HNE1 and primary nasopharyngeal carcinoma samples. Conclusions: We cloned a novel gene UBAP1, which is highly conserved between human and mouse. Clearly, as a novel member of UBA domain protein family and taking its map location into account, a more extensive analysis is essential to establish whether subtle mutations are present in nasopharyngeal carcinomas.

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Documento generato il 25/09/20 alle ore 00:15:21